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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1993-8-31
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pubmed:abstractText |
Alveolar macrophages (AM) produce various inflammatory and immunomodulatory cytokines. The objective of these experiments was to evaluate the production of IL-1ra, a specific receptor antagonist of IL-1, by AM from nonsmoking control subjects (n = 9), smoking control subjects (n = 6), and patients with interstitial lung disease (ILD) (n = 9). IL-1ra protein levels in cultured AM lysates and supernatants were determined by a specific ELISA; relative steady-state IL-1ra mRNA levels were measured using a specific cDNA probe. Before culture the isolated AM from all subject groups contained undetectable IL-1ra mRNA and no IL-1ra protein in the cell lysates as determined by ELISA. AM from nonsmoking control subjects spontaneously produced IL-1ra protein after a 20 h culture in medium, approximately 12 ng/ml with around half in cell lysates. AM from smoking control subjects produced levels of IL-1ra that were similar to the levels in AM from nonsmokers. In contrast, AM from nonsmoking ILD patients (n = 6) produced high levels of IL-1ra spontaneously (approximately 28 ng/ml), with no enhancement observed when cultured on adherent IgG. Interestingly, AM from smoking ILD patients (n = 3) produced lower levels of IL-1ra protein (approximately 11 ng/ml) that were comparable to levels noted in smoking control subjects. AM from all three types of subjects produced decreased amounts of IL-1ra in response to LPS and enhanced amounts in response to GM-CSF. In general, IL-1ra steady-state mRNA levels correlated with protein production.(ABSTRACT TRUNCATED AT 250 WORDS)
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
AIM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0003-0805
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
148
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
495-503
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:8342915-Adult,
pubmed-meshheading:8342915-Bronchoalveolar Lavage Fluid,
pubmed-meshheading:8342915-Cell Adhesion,
pubmed-meshheading:8342915-Cell Division,
pubmed-meshheading:8342915-Female,
pubmed-meshheading:8342915-Granulocyte-Macrophage Colony-Stimulating Factor,
pubmed-meshheading:8342915-Humans,
pubmed-meshheading:8342915-Lipopolysaccharides,
pubmed-meshheading:8342915-Macrophages, Alveolar,
pubmed-meshheading:8342915-Male,
pubmed-meshheading:8342915-Middle Aged,
pubmed-meshheading:8342915-Protein Biosynthesis,
pubmed-meshheading:8342915-Pulmonary Fibrosis,
pubmed-meshheading:8342915-RNA, Messenger,
pubmed-meshheading:8342915-Receptors, Interleukin-1,
pubmed-meshheading:8342915-Smoking,
pubmed-meshheading:8342915-T-Lymphocytes
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pubmed:year |
1993
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pubmed:articleTitle |
Enhanced production of IL-1 receptor antagonist by alveolar macrophages from patients with interstitial lung disease.
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pubmed:affiliation |
Rheumatology Section, Denver VA Medical Center (111G), CO 80220.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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