Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
1993-9-2
|
| pubmed:abstractText |
Sex and the kappa-casein genotype of in vitro produced cattle demiembryos was identified by simultaneous PCR-genotyping using polymorphic sites in ZFY/ZFX and kappa-casein genes. We obtained PCR products of 530 bp (kappa-casein) and of 445/447 bp (sex). Sex and the two kappa-casein alleles could be identified after digestion of the PCR products with Pstl and simultaneous separation of the restriction fragments on one step, allowing a rapid and efficient analyses of embryos.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
0514-7158
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
40
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
307-9
|
| pubmed:dateRevised |
2010-11-18
|
| pubmed:meshHeading |
pubmed-meshheading:8342362-Animals,
pubmed-meshheading:8342362-Base Sequence,
pubmed-meshheading:8342362-Caseins,
pubmed-meshheading:8342362-Cattle,
pubmed-meshheading:8342362-DNA, Single-Stranded,
pubmed-meshheading:8342362-Fertilization in Vitro,
pubmed-meshheading:8342362-Genotype,
pubmed-meshheading:8342362-Molecular Sequence Data,
pubmed-meshheading:8342362-Polymerase Chain Reaction,
pubmed-meshheading:8342362-Sex Determination Analysis
|
| pubmed:year |
1993
|
| pubmed:articleTitle |
Simultaneous genotyping of sex and kappa-casein of bovine in vitro fertilized embryos by the PCR technique.
|
| pubmed:affiliation |
Institute of Animal Breeding and Genetics, Veterinary University, Vienna, Austria.
|
| pubmed:publicationType |
Journal Article
|