|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
14
|
|
pubmed:dateCreated |
1993-8-30
|
|
pubmed:databankReference |
|
|
pubmed:abstractText |
DNA cloned from the marine bacterium Vibrio vulnificus into Escherichia coli HB101 can hydrolyze chitin oligomer analogs in the recipient. The nucleotide sequence of the cloned DNA was determined and a single long open reading frame of 2541 base pairs (initiation codon through termination codon) was found. The nucleotide sequence predicts a gene product of 847 amino acids and a molecular mass of 94.3 kDa. In vitro transcription and translation analyses indicated a single protein of 94 kDa encoded by the cloned DNA. The gene product hydrolyzes methylumbelliferyl beta-D conjugates of chitotriose, chitobiose, N-acetylglucosamine, and N-acetylgalactosamine and has, therefore, been termed a beta-N-acetylhexosaminidase. The predicted protein shares a high degree of sequence similarity with the chitobiase of Vibrio harveyi and limited similarity with the alpha chain of human beta-hexosaminidase. Cluster analyses suggest a common evolutionary ancestor for all known hexosaminidase enzymes, with no detectable relationship to known chitinases.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8341694-14908027,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8341694-16560102,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8341694-2579389,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8341694-2670926,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8341694-271968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8341694-2933746,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8341694-2964638,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8341694-2971567,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8341694-2972716,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8341694-2977375,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8341694-2983426,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8341694-2985470,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8341694-3013851,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8341694-3474623,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8341694-3524459,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8341694-3662513,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8341694-4894282,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8341694-5420325,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8341694-6323249,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8341694-6572363
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jul
|
|
pubmed:issn |
0027-8424
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
15
|
|
pubmed:volume |
90
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
6751-5
|
|
pubmed:dateRevised |
2010-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:8341694-Amino Acid Sequence,
pubmed-meshheading:8341694-Base Sequence,
pubmed-meshheading:8341694-Biological Evolution,
pubmed-meshheading:8341694-Cloning, Molecular,
pubmed-meshheading:8341694-Cluster Analysis,
pubmed-meshheading:8341694-Genes, Bacterial,
pubmed-meshheading:8341694-Hexosaminidases,
pubmed-meshheading:8341694-Molecular Sequence Data,
pubmed-meshheading:8341694-Regulatory Sequences, Nucleic Acid,
pubmed-meshheading:8341694-Restriction Mapping,
pubmed-meshheading:8341694-Sequence Analysis, DNA,
pubmed-meshheading:8341694-Sequence Homology, Amino Acid,
pubmed-meshheading:8341694-Vibrio,
pubmed-meshheading:8341694-beta-N-Acetylhexosaminidases
|
|
pubmed:year |
1993
|
|
pubmed:articleTitle |
Sequence analysis of the beta-N-acetylhexosaminidase gene of Vibrio vulnificus: evidence for a common evolutionary origin of hexosaminidases.
|
|
pubmed:affiliation |
University of Maryland, Center of Marine Biotechnology, Maryland Biotechnology Institute, Baltimore 20742.
|
|
pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, Non-P.H.S.
|
