Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
14
pubmed:dateCreated
1993-8-30
pubmed:abstractText
The transactivator of transcription, Tat, of human immunodeficiency virus type 1 (HIV-1) is required for viral replication. Inhibition of Tat function could have the potential to keep integrated provirus in dormancy. In the presence of Tat, Ro 24-7429, an analog of Ro 5-3335, inhibited expression of indicator genes controlled by the HIV-1 long terminal repeat promoter in transient transfection assays and in a constitutive cell line at noncytotoxic concentrations. Reduction of steady-state mRNA of the indicator gene by the compound correlated with reduction of the gene product in the constitutive cell line. Ro 24-7429 has broad activity against several strains of HIV-1 in different cell lines, peripheral blood lymphocytes, and macrophages (IC90 = 1-3 microM). Importantly, Ro 24-7429 inhibited viral replication in both acute and chronic infection in vitro, a characteristic expected of a Tat antagonist and not shared by viral reverse transcriptase inhibitors. Consistent with this, the compound reduced cell-associated viral RNA and proteins and partially restored cell-surface CD4 in chronically infected cells. After 2 years of continued weekly passage of the virus in fresh CEM cells grown in the presence of the compound at 1 or 10 microM, the virus did not develop resistance to the drug. These results indicate that the compound's action might involve a cellular factor.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/8341644-1282790, http://linkedlifedata.com/resource/pubmed/commentcorrection/8341644-1377363, http://linkedlifedata.com/resource/pubmed/commentcorrection/8341644-1404619, http://linkedlifedata.com/resource/pubmed/commentcorrection/8341644-1457403, http://linkedlifedata.com/resource/pubmed/commentcorrection/8341644-1559613, http://linkedlifedata.com/resource/pubmed/commentcorrection/8341644-1658792, http://linkedlifedata.com/resource/pubmed/commentcorrection/8341644-1698205, http://linkedlifedata.com/resource/pubmed/commentcorrection/8341644-1714522, http://linkedlifedata.com/resource/pubmed/commentcorrection/8341644-1715516, http://linkedlifedata.com/resource/pubmed/commentcorrection/8341644-1716788, http://linkedlifedata.com/resource/pubmed/commentcorrection/8341644-1722324, http://linkedlifedata.com/resource/pubmed/commentcorrection/8341644-1763331, http://linkedlifedata.com/resource/pubmed/commentcorrection/8341644-2023953, http://linkedlifedata.com/resource/pubmed/commentcorrection/8341644-2033665, http://linkedlifedata.com/resource/pubmed/commentcorrection/8341644-2169351, http://linkedlifedata.com/resource/pubmed/commentcorrection/8341644-2184372, http://linkedlifedata.com/resource/pubmed/commentcorrection/8341644-2194290, http://linkedlifedata.com/resource/pubmed/commentcorrection/8341644-2200801, http://linkedlifedata.com/resource/pubmed/commentcorrection/8341644-2205002, http://linkedlifedata.com/resource/pubmed/commentcorrection/8341644-2214026, http://linkedlifedata.com/resource/pubmed/commentcorrection/8341644-2225077, http://linkedlifedata.com/resource/pubmed/commentcorrection/8341644-2227414, http://linkedlifedata.com/resource/pubmed/commentcorrection/8341644-2243395, http://linkedlifedata.com/resource/pubmed/commentcorrection/8341644-2247474, http://linkedlifedata.com/resource/pubmed/commentcorrection/8341644-2420471, http://linkedlifedata.com/resource/pubmed/commentcorrection/8341644-2466937, http://linkedlifedata.com/resource/pubmed/commentcorrection/8341644-2467383, http://linkedlifedata.com/resource/pubmed/commentcorrection/8341644-2470647, http://linkedlifedata.com/resource/pubmed/commentcorrection/8341644-2476805, http://linkedlifedata.com/resource/pubmed/commentcorrection/8341644-2546679, http://linkedlifedata.com/resource/pubmed/commentcorrection/8341644-2550139, http://linkedlifedata.com/resource/pubmed/commentcorrection/8341644-2553266, http://linkedlifedata.com/resource/pubmed/commentcorrection/8341644-2556795, http://linkedlifedata.com/resource/pubmed/commentcorrection/8341644-2825027, http://linkedlifedata.com/resource/pubmed/commentcorrection/8341644-2828663, http://linkedlifedata.com/resource/pubmed/commentcorrection/8341644-2841583, http://linkedlifedata.com/resource/pubmed/commentcorrection/8341644-2849510, http://linkedlifedata.com/resource/pubmed/commentcorrection/8341644-2969774, http://linkedlifedata.com/resource/pubmed/commentcorrection/8341644-2981427, http://linkedlifedata.com/resource/pubmed/commentcorrection/8341644-2991915, http://linkedlifedata.com/resource/pubmed/commentcorrection/8341644-3007995, http://linkedlifedata.com/resource/pubmed/commentcorrection/8341644-3031512, http://linkedlifedata.com/resource/pubmed/commentcorrection/8341644-3172338, http://linkedlifedata.com/resource/pubmed/commentcorrection/8341644-3277284, http://linkedlifedata.com/resource/pubmed/commentcorrection/8341644-3386755, http://linkedlifedata.com/resource/pubmed/commentcorrection/8341644-3417148, http://linkedlifedata.com/resource/pubmed/commentcorrection/8341644-3422113, http://linkedlifedata.com/resource/pubmed/commentcorrection/8341644-3486233, http://linkedlifedata.com/resource/pubmed/commentcorrection/8341644-3492810, http://linkedlifedata.com/resource/pubmed/commentcorrection/8341644-3530501, http://linkedlifedata.com/resource/pubmed/commentcorrection/8341644-6302308, http://linkedlifedata.com/resource/pubmed/commentcorrection/8341644-7332929
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
90
pubmed:owner
NLM
pubmed:authorsComplete
N
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