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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1993-9-2
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pubmed:abstractText |
Gentamycin was determined by solid antigen competition inhibition method of chemiluminescet immunoassay, in which luminol-(p-hydroxydiphenyl)-H2O2 was used a the chemiluminescence system and p-hydroxydiphenyl as the chemiluminescent enhancer. Solid antigen was synthesized by a method in which gentamycin was immobilized covalently on microcrystalline cellulose previously activated by 1,1'-carbonyldiimidazole. The rabbit anti-gentamycin IgG and the HRP-donkey anti-rabbit IgG were used as the first antibody and labelled second antibody, respectively. The linear range of the gentamycin (with blank serum) was 0.03-1 micrograms/tube and the correlation coefficient of the log-log regression equation [r[ was 0.981 +/- 0.016 with a limit of detection 3.3-11.4 ng/tube and the recovery of gentamycin 88.2 +/- 4.5%.
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pubmed:language |
chi
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/4-phenylphenol,
http://linkedlifedata.com/resource/pubmed/chemical/Biphenyl Compounds,
http://linkedlifedata.com/resource/pubmed/chemical/Gentamicins,
http://linkedlifedata.com/resource/pubmed/chemical/Imidazoles,
http://linkedlifedata.com/resource/pubmed/chemical/Luminol,
http://linkedlifedata.com/resource/pubmed/chemical/N,N-carbonyldiimidazole
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0257-7712
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
24
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
101-3
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading | |
pubmed:year |
1993
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pubmed:articleTitle |
[Study on determination of gentamycin by enhanced chemiluminescent immunoassay].
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pubmed:publicationType |
Journal Article,
English Abstract
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