Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
6
|
pubmed:dateCreated |
1993-8-27
|
pubmed:abstractText |
1. Relaxations of strips of rat gastric fundus were elicited with nicotine (100 mumol/L), nitric oxide (NO; 30 mumol/L), sodium nitroprusside (SNP; 100 nmol/L) and vasoactive intestinal polypeptide (VIP; 1 nmol/L). 2. Methylene blue (30 mumol/L), an inhibitor of soluble guanylate cyclase, reduced relaxations elicited by NO and nicotine, but not those elicited by VIP. 3. Chymotrypsin (1 U/mL) abolished VIP-induced relaxations and reduced nicotine-induced relaxations, but had no effect on SNP-induced relaxations. 4. NG-nitro-L-arginine methyl ester (L-NAME; 100 mumol/L), an inhibitor of NO synthase, reduced relaxations elicited by nicotine, but not those elicited by SNP or VIP. 5. When nicotine-induced relaxations had been reduced by either L-NAME or chymotrypsin, the addition of the other agent produced a greater reduction. However, the relaxations were not abolished. 6. Nicotine-induced relaxations were abolished by tetrodotoxin (1 mumol/L) or hexamethonium (100 mumol/L), indicating that they were due to activation of neuronal nicotinic receptors. Their reduction by methylene blue and L-NAME indicates that an NO-like mediator was involved. Their reduction by chymotrypsin indicates that a VIP-like peptide was involved. However, since they were not abolished by a combination of L-NAME and chymotrypsin, it appears that at least one more as yet unidentified mediator may be involved.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Arginine,
http://linkedlifedata.com/resource/pubmed/chemical/Chymotrypsin,
http://linkedlifedata.com/resource/pubmed/chemical/Methylene Blue,
http://linkedlifedata.com/resource/pubmed/chemical/NG-Nitroarginine Methyl Ester,
http://linkedlifedata.com/resource/pubmed/chemical/Nicotine,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide,
http://linkedlifedata.com/resource/pubmed/chemical/Nitroprusside,
http://linkedlifedata.com/resource/pubmed/chemical/Vasoactive Intestinal Peptide
|
pubmed:status |
MEDLINE
|
pubmed:month |
Jun
|
pubmed:issn |
0305-1870
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
20
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
451-7
|
pubmed:dateRevised |
2006-11-15
|
pubmed:meshHeading |
pubmed-meshheading:8339469-Animals,
pubmed-meshheading:8339469-Arginine,
pubmed-meshheading:8339469-Chymotrypsin,
pubmed-meshheading:8339469-Female,
pubmed-meshheading:8339469-Gastric Fundus,
pubmed-meshheading:8339469-Male,
pubmed-meshheading:8339469-Methylene Blue,
pubmed-meshheading:8339469-Muscle, Smooth,
pubmed-meshheading:8339469-Muscle Relaxation,
pubmed-meshheading:8339469-NG-Nitroarginine Methyl Ester,
pubmed-meshheading:8339469-Nicotine,
pubmed-meshheading:8339469-Nitric Oxide,
pubmed-meshheading:8339469-Nitroprusside,
pubmed-meshheading:8339469-Rats,
pubmed-meshheading:8339469-Rats, Sprague-Dawley,
pubmed-meshheading:8339469-Vasoactive Intestinal Peptide
|
pubmed:year |
1993
|
pubmed:articleTitle |
Mediators of nicotine-induced relaxations of the rat gastric fundus.
|
pubmed:affiliation |
Department of Pharmacology, University of Melbourne, Parkville, Victoria, Australia.
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|