Linked Life Data

8337234

Source:http://linkedlifedata.com/resource/pubmed/id/8337234

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1993-8-23
pubmed:abstractText
The present investigation was undertaken to clarify the interaction of beta-alanyl-L-histidinato zinc (AHZ) and other bone-regulating factors on bone alkaline phosphatase in tissue culture. Calvariae were removed from weanling rats (3-week-old males) and cultured for periods up to 48 h in Dulbecco's modified Eagle medium. The experimental cultures contained 10(-5) mol/l AHZ which reveals in maximum effect on bone formation. Bone alkaline phosphatase activity was significantly increased by the presence of AHZ (10(-5) mol/l), insulin (10(-8) mol/l), sodium fluoride (10(-2) mol/l), aluminium sulfate (2 x 10(-3) mol/l), while the enzyme activity was not altered by estradiol (10(-9) mol/l), calcitonin (3 x 10(-8) mol/l), hydrocortisone (10(-8) mol/l), indomethacin (10(-6) mol/l) and imidazole (10(-3) mol/l). The presence of AHZ (10(-5) mol/l) clearly enhanced the calcitonin-, sodium fluoride- or diltiazem-increased bone alkaline phosphatase activity. Meanwhile, AHZ did not interact for the effects of other hormones and reagents. Moreover, the presence of cycloheximide (10(-6) mol/l), an inhibitor of protein synthesis, completely blocked the enhancement of bone alkaline phosphatase activity by AHZ. These findings suggest that AHZ has an effect different from bone cellular response for other bone-regulating factors, and that bone protein synthesis was a necessary component for AHZ action.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0031-7012
pubmed:author
pubmed:issnType
Print
pubmed:volume
47
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
66-72
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Beta-alanyl-L-histidinato zinc enhances various bone-regulating factors' effects on bone alkaline phosphatase activity in tissue culture.
pubmed:affiliation
Laboratory of Metabolism and Endocrinology, Graduate School of Nutritional Sciences, University of Shizuoka, Japan.
pubmed:publicationType
Journal Article