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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1993-8-24
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pubmed:databankReference | |
pubmed:abstractText |
Heterotrimeric G proteins consisting of alpha, beta, and gamma subunits couple sensory, hormone, and neurotransmitter receptors to intracellular and transmembrane effectors. Several splicing variants of the GS (the G protein that stimulates adenylyl cyclase) alpha subunit (GS alpha) have been described. Some of these couple receptors to stimulation of adenylyl cyclase and Ca2+ channels, whereas others encode truncated proteins whose functions are not currently defined. We describe a 1321N1 human astrocytoma cDNA clone for a novel GS alpha isoform isolated from astrocytoma cells (G(astro)) that is identical to GS alpha-1 with the exception of a novel 5' sequence extending into the previously described exon 1 of GS alpha, a single base change, and an alternative polyadenylation site. Analysis by northern blotting and reverse transcription/PCR confirms the presence of an mRNA corresponding to this cDNA in astrocytoma cells. Additional northern analysis indicates that G(astro) recognizes two novel GS alpha mRNAs in the rat: a 2.0-kb mRNA expressed only in neural and neuroendocrine tissues and a 1.8-kb mRNA that is ubiquitously expressed. Functional analysis of G(astro) is complicated by the apparent insertion of alphoid satellite DNA into the transcription unit. The resulting cDNA encodes a truncated protein that may be translated from the methionine in exon 2 as previously described.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0022-3042
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
61
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
712-7
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:8336149-Amino Acid Sequence,
pubmed-meshheading:8336149-Animals,
pubmed-meshheading:8336149-Astrocytoma,
pubmed-meshheading:8336149-Base Sequence,
pubmed-meshheading:8336149-Blotting, Northern,
pubmed-meshheading:8336149-DNA,
pubmed-meshheading:8336149-GTP-Binding Proteins,
pubmed-meshheading:8336149-Humans,
pubmed-meshheading:8336149-Molecular Sequence Data,
pubmed-meshheading:8336149-Polymerase Chain Reaction,
pubmed-meshheading:8336149-RNA, Messenger,
pubmed-meshheading:8336149-Rats,
pubmed-meshheading:8336149-Ribonucleases,
pubmed-meshheading:8336149-Sequence Analysis,
pubmed-meshheading:8336149-Tumor Cells, Cultured
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pubmed:year |
1993
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pubmed:articleTitle |
Isolation and characterization of a novel cDNA which identifies both neural-specific and ubiquitously expressed GS alpha mRNAs.
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pubmed:affiliation |
Department of Pharmacology, University of Washington School of Medicine, Seattle 98195.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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