Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:dateCreated |
1993-8-25
|
| pubmed:abstractText |
A previous study of the 12 nucleotide-long influenza A virion RNA promoter has shown that three nucleotides, residues 9 to 11, were crucial for transcription in vitro, although other nucleotides play a significant but less important role. A model for polymerase-promoter recognition was proposed, according to which there were two sites: a binding site at residues 9 to 11 and a regulatory site at or near the site of initiation at residue 1. By studying the effect of point mutations in the promoter on the binding efficiency of the polymerase using a photochemical cross-linking assay, we now show that residues 9 to 12 are crucial for binding. In addition residues 4 to 8, though not as important, are involved in binding, possibly by stabilizing the polymerase-promoter complex. Both PB1 and PB2 apparently play an important role during virion RNA promoter recognition and binding.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jul
|
| pubmed:issn |
0022-1317
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
74 ( Pt 7)
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1327-33
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:8336121-Animals,
pubmed-meshheading:8336121-Base Sequence,
pubmed-meshheading:8336121-Binding, Competitive,
pubmed-meshheading:8336121-Binding Sites,
pubmed-meshheading:8336121-Conserved Sequence,
pubmed-meshheading:8336121-Cross-Linking Reagents,
pubmed-meshheading:8336121-DNA-Directed RNA Polymerases,
pubmed-meshheading:8336121-Influenza A virus,
pubmed-meshheading:8336121-Kinetics,
pubmed-meshheading:8336121-Molecular Sequence Data,
pubmed-meshheading:8336121-Mutagenesis, Insertional,
pubmed-meshheading:8336121-Mutagenesis, Site-Directed,
pubmed-meshheading:8336121-Photochemistry,
pubmed-meshheading:8336121-Point Mutation,
pubmed-meshheading:8336121-Promoter Regions, Genetic,
pubmed-meshheading:8336121-RNA, Viral,
pubmed-meshheading:8336121-Rabbits,
pubmed-meshheading:8336121-Templates, Genetic,
pubmed-meshheading:8336121-Virion
|
| pubmed:year |
1993
|
| pubmed:articleTitle |
Photochemical cross-linking of influenza A polymerase to its virion RNA promoter defines a polymerase binding site at residues 9 to 12 of the promoter.
|
| pubmed:affiliation |
Chemical Pathology Unit, Sir William Dunn School of Pathology, University of Oxford, U.K.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|