| pubmed-article:8335925 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8335925 | lifeskim:mentions | umls-concept:C0079633 | lld:lifeskim |
| pubmed-article:8335925 | lifeskim:mentions | umls-concept:C0128897 | lld:lifeskim |
| pubmed-article:8335925 | lifeskim:mentions | umls-concept:C0301872 | lld:lifeskim |
| pubmed-article:8335925 | lifeskim:mentions | umls-concept:C0033268 | lld:lifeskim |
| pubmed-article:8335925 | lifeskim:mentions | umls-concept:C1880022 | lld:lifeskim |
| pubmed-article:8335925 | lifeskim:mentions | umls-concept:C1515895 | lld:lifeskim |
| pubmed-article:8335925 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:8335925 | pubmed:dateCreated | 1993-8-26 | lld:pubmed |
| pubmed-article:8335925 | pubmed:abstractText | Delayed-type hypersensitivity and allograft rejection are dependent upon the generation of Ag-specific T cell immune responses. The mixed lymphocyte reaction is a model of alloantigen-driven immunity and has provided significant insight into the mechanisms of T cell proliferation. Recently, soluble mediators, including TNF-alpha have been shown to be involved in the full expression of this response. In order to elucidate the potential mechanisms of cellular recruitment in the context of either delayed-type hypersensitivity or allograft rejection, we employed a mixed lymphocyte reaction to study the production of chemokines, monocyte chemoattractant protein-1 (MCP-1), and IL-8. Time-course experiments demonstrated that significant levels of MCP-1 and IL-8 were produced in allogeneic cultures as early as day 1, and that this relationship persisted over 6 days in culture. Northern blot analysis of chemokine mRNA confirmed the early induction of these genes in the allogeneic response. The levels of MCP-1 and IL-8 protein from mixed lymphocyte reaction supernatants as measured by specific ELISA were positively correlated with the proliferative response as measured by [3H]TdR uptake. Although significant MCP-1 and IL-8 were produced during a mixed lymphocyte reaction, these molecules did not participate in the proliferative response, as neutralizing antibodies to either MCP-1 or IL-8 had no effect on proliferation. In order to ascertain the mechanism for the induction of MCP-1 and IL-8 during the mixed lymphocyte reaction, experiments were performed in the presence of neutralizing anti-human TNF antibodies. Neutralization of TNF resulted in significant abrogation of MCP-1 and IL-8 production (75 +/- 5 and 87 +/- 2 percent, respectively). Cells isolated from the mixed lymphocyte reaction at day 6, demonstrated that MCP-1 and IL-8 Ag were localized to mononuclear phagocytes. These results demonstrate that potent chemokines, MCP-1 and IL-8, are produced during the evolution of a mixed lymphocyte reaction, and their induction is TNF-dependent. | lld:pubmed |
| pubmed-article:8335925 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8335925 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8335925 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8335925 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8335925 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8335925 | pubmed:citationSubset | AIM | lld:pubmed |
| pubmed-article:8335925 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8335925 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8335925 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8335925 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8335925 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8335925 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8335925 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8335925 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:8335925 | pubmed:issn | 0022-1767 | lld:pubmed |
| pubmed-article:8335925 | pubmed:author | pubmed-author:ToewsG BGB | lld:pubmed |
| pubmed-article:8335925 | pubmed:author | pubmed-author:ChristensenP... | lld:pubmed |
| pubmed-article:8335925 | pubmed:author | pubmed-author:StrieterR MRM | lld:pubmed |
| pubmed-article:8335925 | pubmed:author | pubmed-author:BurdickM DMD | lld:pubmed |
| pubmed-article:8335925 | pubmed:author | pubmed-author:StandifordT... | lld:pubmed |
| pubmed-article:8335925 | pubmed:author | pubmed-author:RolfeM WMW | lld:pubmed |
| pubmed-article:8335925 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8335925 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:8335925 | pubmed:volume | 151 | lld:pubmed |
| pubmed-article:8335925 | pubmed:geneSymbol | IL-8 | lld:pubmed |
| pubmed-article:8335925 | pubmed:geneSymbol | MCP-1 | lld:pubmed |
| pubmed-article:8335925 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8335925 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8335925 | pubmed:pagination | 1205-13 | lld:pubmed |
| pubmed-article:8335925 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:8335925 | pubmed:meshHeading | pubmed-meshheading:8335925-... | lld:pubmed |
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| pubmed-article:8335925 | pubmed:meshHeading | pubmed-meshheading:8335925-... | lld:pubmed |
| pubmed-article:8335925 | pubmed:meshHeading | pubmed-meshheading:8335925-... | lld:pubmed |
| pubmed-article:8335925 | pubmed:meshHeading | pubmed-meshheading:8335925-... | lld:pubmed |
| pubmed-article:8335925 | pubmed:meshHeading | pubmed-meshheading:8335925-... | lld:pubmed |
| pubmed-article:8335925 | pubmed:meshHeading | pubmed-meshheading:8335925-... | lld:pubmed |
| pubmed-article:8335925 | pubmed:meshHeading | pubmed-meshheading:8335925-... | lld:pubmed |
| pubmed-article:8335925 | pubmed:meshHeading | pubmed-meshheading:8335925-... | lld:pubmed |
| pubmed-article:8335925 | pubmed:year | 1993 | lld:pubmed |
| pubmed-article:8335925 | pubmed:articleTitle | Characterization of the production of monocyte chemoattractant protein-1 and IL-8 in an allogeneic immune response. | lld:pubmed |
| pubmed-article:8335925 | pubmed:affiliation | Department of Internal Medicine, University of Michigan Medical Center, Ann Arbor 48109-0360. | lld:pubmed |
| pubmed-article:8335925 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8335925 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:8335925 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| pubmed-article:8335925 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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