Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1993-8-23
pubmed:abstractText
Eosinophil major basic protein (MBP), purified from guinea pig eosinophil granules was used to raise five monoclonal antibodies (MoAbs). Their reactivity with MBP was confirmed by immunoblotting and indirect ELISA. Two of the MoAbs were used to develop a sensitive and specific antigen capture (sandwich) ELISA for guinea pig eosinophil MBP which gives an accurate and reproducible standard curve over the range of 10-10,000 ng/ml. The specificity of the ELISA for MBP was confirmed and its suitability for testing biological samples ascertained by measurement of MBP in bronchoalveolar lavage fluid (BALF) and plasma from guinea pigs sensitized and challenged with ovalbumin. The ELISA was also capable of detecting MBP in culture supernatants from purified eosinophil preparations challenged with calcium ionophore in vitro. One of the monoclonals could be used to strongly and specifically stain guinea pig eosinophils in immunocytochemistry, whilst all five could be used to visualize eosinophils in suspension in BALF or peritoneal lavage fluid by flow cytometry. There was no staining of other guinea pig leucocyte types, nor crossreactivity with human eosinophils by immunocytochemistry or flow cytometry.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0954-7894
pubmed:author
pubmed:issnType
Print
pubmed:volume
23
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
425-34
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed-meshheading:8334540-Animals, pubmed-meshheading:8334540-Antibodies, Monoclonal, pubmed-meshheading:8334540-Antibody Specificity, pubmed-meshheading:8334540-Blood Proteins, pubmed-meshheading:8334540-Bronchoalveolar Lavage Fluid, pubmed-meshheading:8334540-Cross Reactions, pubmed-meshheading:8334540-Enzyme-Linked Immunosorbent Assay, pubmed-meshheading:8334540-Eosinophil Granule Proteins, pubmed-meshheading:8334540-Eosinophils, pubmed-meshheading:8334540-Flow Cytometry, pubmed-meshheading:8334540-Guinea Pigs, pubmed-meshheading:8334540-Humans, pubmed-meshheading:8334540-Immunoenzyme Techniques, pubmed-meshheading:8334540-Mice, pubmed-meshheading:8334540-Ovalbumin, pubmed-meshheading:8334540-Rabbits, pubmed-meshheading:8334540-Reproducibility of Results, pubmed-meshheading:8334540-Ribonucleases, pubmed-meshheading:8334540-Sensitivity and Specificity
pubmed:year
1993
pubmed:articleTitle
Monoclonal antibodies specific for guinea pig eosinophil major basic protein: their use in an ELISA, immunocytochemistry and flow cytometry.
pubmed:affiliation
Immunopharmacology Group, Southampton General Hospital, U.K.
pubmed:publicationType
Journal Article