rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1993-8-17
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pubmed:abstractText |
With regard to the expression of adhesion molecules, human myeloma cells freshly isolated from bone marrow were heterogeneous. By two-color analysis with anti-VLA-5 antibody (PE staining) and FITC-labeled anti-CD38 antibody, we found all myeloma cells located at CD38-strong positive (CD38++) fraction and identified two subpopulations among these myeloma cells: CD38++ VLA-5-(VLA-5-) myeloma cells and CD38++ VLA-5+ (VLA-5+) myeloma cells. To clarify the biologic character of these two subpopulations, the morphology, in vitro proliferative activity and in vitro M-protein secretion were examined in each fraction isolated by the purification procedure or a cell sorter. Morphologic examination showed that VLA-5- myeloma cells were mostly immature or plasmablastic and VLA-5+ cells were mature myeloma cells. Furthermore, VLA-5- myeloma cells proliferated markedly in vitro and responded to interleukin 6 (IL-6), a growth factor for myeloma cells, while VLA-5+ myeloma cells showed very low uptakes of 3H-thymidine and no responses to IL-6 but secreted higher amounts of M-protein (immunoglobulin) in vitro significantly. Therefore, we could clarify here heterogeneity of human myeloma cells in the bone marrow with regard to the expression of VLA-5, one of integrin adhesion molecules; VLA-5- myeloma cells were proliferative immature cells and VLA-5+ cells were mature myeloma cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/ADP-ribosyl Cyclase,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD38,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation,
http://linkedlifedata.com/resource/pubmed/chemical/CD38 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin G,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Fibronectin,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Very Late Antigen
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0006-4971
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
82
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
564-70
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:8329711-ADP-ribosyl Cyclase,
pubmed-meshheading:8329711-Antigens, CD,
pubmed-meshheading:8329711-Antigens, CD38,
pubmed-meshheading:8329711-Antigens, Differentiation,
pubmed-meshheading:8329711-Base Sequence,
pubmed-meshheading:8329711-Bone Marrow,
pubmed-meshheading:8329711-Cell Division,
pubmed-meshheading:8329711-Clone Cells,
pubmed-meshheading:8329711-DNA, Neoplasm,
pubmed-meshheading:8329711-Humans,
pubmed-meshheading:8329711-Immunoglobulin G,
pubmed-meshheading:8329711-Immunophenotyping,
pubmed-meshheading:8329711-Membrane Glycoproteins,
pubmed-meshheading:8329711-Molecular Sequence Data,
pubmed-meshheading:8329711-Multiple Myeloma,
pubmed-meshheading:8329711-Plasma Cells,
pubmed-meshheading:8329711-Polymerase Chain Reaction,
pubmed-meshheading:8329711-Receptors, Fibronectin,
pubmed-meshheading:8329711-Receptors, Very Late Antigen
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pubmed:year |
1993
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pubmed:articleTitle |
Identification of immature and mature myeloma cells in the bone marrow of human myelomas.
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pubmed:affiliation |
Department of Internal Medicine, Research Institute for Nuclear Medicine and Biology, Hiroshima University, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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