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PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1993-8-17
pubmed:abstractText
With regard to the expression of adhesion molecules, human myeloma cells freshly isolated from bone marrow were heterogeneous. By two-color analysis with anti-VLA-5 antibody (PE staining) and FITC-labeled anti-CD38 antibody, we found all myeloma cells located at CD38-strong positive (CD38++) fraction and identified two subpopulations among these myeloma cells: CD38++ VLA-5-(VLA-5-) myeloma cells and CD38++ VLA-5+ (VLA-5+) myeloma cells. To clarify the biologic character of these two subpopulations, the morphology, in vitro proliferative activity and in vitro M-protein secretion were examined in each fraction isolated by the purification procedure or a cell sorter. Morphologic examination showed that VLA-5- myeloma cells were mostly immature or plasmablastic and VLA-5+ cells were mature myeloma cells. Furthermore, VLA-5- myeloma cells proliferated markedly in vitro and responded to interleukin 6 (IL-6), a growth factor for myeloma cells, while VLA-5+ myeloma cells showed very low uptakes of 3H-thymidine and no responses to IL-6 but secreted higher amounts of M-protein (immunoglobulin) in vitro significantly. Therefore, we could clarify here heterogeneity of human myeloma cells in the bone marrow with regard to the expression of VLA-5, one of integrin adhesion molecules; VLA-5- myeloma cells were proliferative immature cells and VLA-5+ cells were mature myeloma cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0006-4971
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
82
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
564-70
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:8329711-ADP-ribosyl Cyclase, pubmed-meshheading:8329711-Antigens, CD, pubmed-meshheading:8329711-Antigens, CD38, pubmed-meshheading:8329711-Antigens, Differentiation, pubmed-meshheading:8329711-Base Sequence, pubmed-meshheading:8329711-Bone Marrow, pubmed-meshheading:8329711-Cell Division, pubmed-meshheading:8329711-Clone Cells, pubmed-meshheading:8329711-DNA, Neoplasm, pubmed-meshheading:8329711-Humans, pubmed-meshheading:8329711-Immunoglobulin G, pubmed-meshheading:8329711-Immunophenotyping, pubmed-meshheading:8329711-Membrane Glycoproteins, pubmed-meshheading:8329711-Molecular Sequence Data, pubmed-meshheading:8329711-Multiple Myeloma, pubmed-meshheading:8329711-Plasma Cells, pubmed-meshheading:8329711-Polymerase Chain Reaction, pubmed-meshheading:8329711-Receptors, Fibronectin, pubmed-meshheading:8329711-Receptors, Very Late Antigen
pubmed:year
1993
pubmed:articleTitle
Identification of immature and mature myeloma cells in the bone marrow of human myelomas.
pubmed:affiliation
Department of Internal Medicine, Research Institute for Nuclear Medicine and Biology, Hiroshima University, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't