Linked Life Data

8324919

Source:http://linkedlifedata.com/resource/pubmed/id/8324919

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1993-8-6
pubmed:abstractText
1. Addition of endothelin-1 (ET-1) to [3H]-inositol-labelled neonatal rat hearts stimulated the accumulation of [3H]-labelled inositol phosphates (InsP), but only at high concentrations; concentration at half maximum stimulation (EC50) > 0.1 mumol/L). When similar experiments were performed using isolated myocytes, the potency of endothelin-1 was higher and the EC50 value averaged 3.2 +/- 0.5 nmol/L (mean +/- s.e.m., n = 4). 2. The binding affinity of [125I]-endothelin-1 was higher for receptors on isolated cells than for receptors on membranes prepared from intact heart (72 +/- 16 pmol/L compared with 3.9 +/- 0.7 nmol/L, mean +/- s.e.m., n = 4, P < 0.01; Students' t test). 3. Receptors from both sources were cross-linked to [125I]-endothelin-1 and their molecular weights measured using sodium dodecylsulfate gradient polyacrylamide gel electrophoresis (SDS-PAGE). The receptors present on the isolated cells had a higher molecular weight (48 kD) than the receptor on the heart membranes (38 kD).
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0305-1870
pubmed:author
pubmed:issnType
Print
pubmed:volume
20
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
331-4
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
A low affinity, low molecular weight endothelin-A receptor present in neonatal rat heart.
pubmed:affiliation
Cellular Biochemistry Laboratory, Baker Medical Research Institute, Prahran, Victoria, Australia.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't