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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1993-8-12
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pubmed:abstractText |
A full-length cDNA coding a human beta 2-glycoprotein I (beta 2-GPI) was introduced into the baculovirus genome to construct a recombinant baculovirus. Spodoptera frugiperda (Sf9) cells were infected with the recombinant baculovirus. A protein (mol. wt 43,000) reactive with anti-beta 2-GPI antisera was produced in the insect cells and secreted into the culture medium. The recombinant beta 2-GPI was purified from the culture supernatant by sequential cardiolipin (CL)-affinity column chromatography and gel filtration. The N-terminal amino acid sequence of the protein was identical to that of the native beta 2-GPI purified from human sera, and a putative signal peptide was cleaved from the secreted form of the recombinant protein. The purified recombinant protein had a cofactor activity which enhances CL binding of anticardiolipin antibodies (aCL) in systemic lupus erythematosus (SLE) patients, as well as the native beta 2-GPI. Thus, the beta 2-GPI expressed in insect cells is an immunologically active cofactor.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8324900-1567411,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8324900-1567819,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8324900-1602135,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8324900-1650181,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8324900-1657987,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8324900-16789242,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8324900-1748314,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8324900-1751487,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8324900-1777418,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8324900-1937047,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8324900-1972485,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8324900-1973491,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8324900-1974250,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8324900-222615,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8324900-2349221,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8324900-2751824,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8324900-2844532,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8324900-2960381,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8324900-3553425,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8324900-3768409,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8324900-3827975,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8324900-3878519,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8324900-388439,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8324900-4052628,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8324900-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8324900-6139567,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8324900-6201309,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8324900-6318086,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8324900-6427035,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8324900-6587378,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8324900-942051
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0009-9104
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
93
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
19-25
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pubmed:dateRevised |
2010-9-13
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pubmed:meshHeading |
pubmed-meshheading:8324900-Amino Acid Sequence,
pubmed-meshheading:8324900-Animals,
pubmed-meshheading:8324900-Apolipoproteins,
pubmed-meshheading:8324900-Baculoviridae,
pubmed-meshheading:8324900-Base Sequence,
pubmed-meshheading:8324900-Cardiolipins,
pubmed-meshheading:8324900-Cell Line,
pubmed-meshheading:8324900-Chromatography, Affinity,
pubmed-meshheading:8324900-Chromatography, Gel,
pubmed-meshheading:8324900-Gene Expression Regulation, Viral,
pubmed-meshheading:8324900-Glycoproteins,
pubmed-meshheading:8324900-Humans,
pubmed-meshheading:8324900-Lupus Erythematosus, Systemic,
pubmed-meshheading:8324900-Molecular Sequence Data,
pubmed-meshheading:8324900-Moths,
pubmed-meshheading:8324900-Protein Sorting Signals,
pubmed-meshheading:8324900-Recombinant Proteins,
pubmed-meshheading:8324900-Recombination, Genetic,
pubmed-meshheading:8324900-Transfection,
pubmed-meshheading:8324900-beta 2-Glycoprotein I
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pubmed:year |
1993
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pubmed:articleTitle |
Expression of anticardiolipin cofactor, human beta 2-glycoprotein I, by a recombinant baculovirus/insect cell system.
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pubmed:affiliation |
Microbiology Laboratory, Yamasa Corporation, Choshi, Japan.
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pubmed:publicationType |
Journal Article
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