Linked Life Data

8324893

Source:http://linkedlifedata.com/resource/pubmed/id/8324893

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1993-8-10
pubmed:abstractText
We investigated the effect of platelet-activating factor (PAF) on basal and insulin-stimulated sodium-dependent neutral amino acid transport by system A in rat soleus muscle. Fasted male Sprague-Dawley rats (75-100 g) were given an intravenous injection of saline or PAF (4 micrograms/kg) and killed 1 hr later. Isolated soleus muscles were incubated in media containing 1-14C-alpha-aminoisobutyric acid (AIB; system A amino acid analog), 3H-inulin, and 0 (basal) or 100 mU/ml insulin. Na(+)-free media were assessed by Na(+)-independent AIB uptake. The rate of AIB uptake by muscle was corrected for uptake into extracellular (inulin) space. Basal cellular AIB uptake by soleus muscles from PAF-treated rats was 30.2% lower than in controls. Insulin increased the absolute rate of cellular AIB uptake above basal levels to the same extent in muscles of control and PAF-treated rats, although the rate of maximal insulin-stimulated AIB uptake was 20% lower in muscles of PAF-treated rats. Na(+)-independent AIB uptake (measured in Na(+)-free media) was the same in muscles of control and PAF-treated rats. The fact that AIB uptake in Na(+)-free media was the same in muscles of control and PAF-treated rats indicates that the reduction in basal and insulin-stimulated AIB uptake in muscles of PAF-treated rats was due to an alteration of system A Na(+)-dependent amino acid transport.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0092-6213
pubmed:author
pubmed:issnType
Print
pubmed:volume
40
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
75-80
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Effect of platelet-activating factor on basal and insulin-mediated system A amino acid transport in rat soleus muscle.
pubmed:affiliation
Department of Anesthesiology, Graduate School of Medicine, University of Tennessee Medical Center, Knoxville 37920.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.