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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1993-8-10
|
| pubmed:abstractText |
Cytoplasmic Ca2+ response to flow and its relation to the release of endothelium-derived relaxing factor (EDRF) by endothelial cells were studied in vitro. Intracellular Ca2+ concentration ([Ca2+]i) and EDRF were measured using Fura-2 and photometric fluorescence microscopy, and a bioassay method using a rabbit aortic ring, respectively. Cultured endothelial cells were exposed to flow in Hanks' balanced salt solution containing various concentrations of ATP in a specially designed apparatus. At ATP levels over 100 nM, the application of flow to cells led to an apparent increase in [Ca2+]i and when flow stopped [Ca2+]i returned to the resting level. When the flow rate was increased stepwise, [Ca2+]i increased flow-rate dependently at around 500 nM ATP. At a constant flow rate, endothelial cells were then exposed to the perfusate, the ATP concentration of which was increased stepwise, and the simultaneous changes in [Ca2+]i and release of EDRF were measured. Both [Ca2+]i and EDRF increased as ATP concentrations increased, and there was a proportional relationship between the two. The range of the ATP-induced increase in [Ca2+]i was similar to that of the flow-rate dependent changes observed at 500 nM ATP. These results suggest that vascular endothelial cells have a mechanism by which changes in flow rate or shear stress are recognized at an appropriate concentration of extracellular ATP and the information is converted into changes in [Ca2+]i. Moreover, the flow-induced changes in [Ca2+]i may be involved in the regulation of EDRF release by endothelial cells.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0921-3775
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
5
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
17-21
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading | |
| pubmed:year |
1993
|
| pubmed:articleTitle |
Flow-induced calcium transients and release of endothelium-derived relaxing factor in cultured vascular endothelial cells.
|
| pubmed:affiliation |
Department of Cardiovascular Biomechanics, Faculty of Medicine, University of Tokyo, Japan.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|