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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1993-8-5
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pubmed:databankReference |
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pubmed:abstractText |
We have isolated and sequenced an apparent full-length cDNA clone for phospholipid hydroperoxide glutathione peroxidase (Genbank accession number L12743) from a pig blastocyst cDNA library. The sequence encodes a polypeptide of 170 amino acids, including a TGA-encoded selenocysteine at residue 46, with a calculated M(r) of 19,492 Da. Use of this clone in Northern blot analysis of Se-deficient rat liver revealed that phospholipid hydroperoxide glutathione peroxidase mRNA levels were little affected by Se deficiency, whereas classical glutathione peroxidase mRNA levels were decreased by 90% in the same samples. Lastly, liver phospholipid hydroperoxide glutathione peroxidase mRNA levels were not elevated in female rats, in contrast to classical glutathione peroxidase.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
30
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pubmed:volume |
193
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
905-11
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:8323565-Amino Acid Sequence,
pubmed-meshheading:8323565-Animals,
pubmed-meshheading:8323565-Base Sequence,
pubmed-meshheading:8323565-Blastocyst,
pubmed-meshheading:8323565-Blotting, Northern,
pubmed-meshheading:8323565-DNA,
pubmed-meshheading:8323565-Female,
pubmed-meshheading:8323565-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:8323565-Gene Library,
pubmed-meshheading:8323565-Glutathione Peroxidase,
pubmed-meshheading:8323565-Liver,
pubmed-meshheading:8323565-Male,
pubmed-meshheading:8323565-Molecular Sequence Data,
pubmed-meshheading:8323565-Myocardium,
pubmed-meshheading:8323565-Oligonucleotide Probes,
pubmed-meshheading:8323565-RNA, Messenger,
pubmed-meshheading:8323565-Rats,
pubmed-meshheading:8323565-Selenium,
pubmed-meshheading:8323565-Sequence Homology, Nucleic Acid,
pubmed-meshheading:8323565-Swine
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pubmed:year |
1993
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pubmed:articleTitle |
Phospholipid hydroperoxide glutathione peroxidase: full-length pig blastocyst cDNA sequence and regulation by selenium status.
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pubmed:affiliation |
Dept. of Food Science & Human Nutrition, College of Human Environmental Sciences, University of Missouri, Columbia 65211.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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