Linked Life Data

8323537

Source:http://linkedlifedata.com/resource/pubmed/id/8323537

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1993-8-5
pubmed:abstractText
The ability of glycated serum proteins, which exist in vivo predominantly as Amadori adducts, to influence cell biology suggests the existence of cell binding proteins that recognize glucose adducts in nonenzymatically glycated proteins. To explore this possibility, we applied detergent extracts of aortic endothelial cells to HPLC and to an affinity column of glycated human albumin immobilized onto Sepharose, which was eluted with alkaline pH. HPLC fractionation revealed a peak reactive in ELISA with glycated but not nonglycated albumin. Two polypeptides of approximately 110 kDa and 205 kDa were identified when the affinity bound proteins were electrophoresed, transferred to immunoblotting membranes, and reacted with an enzyme conjugate of glycated albumin containing Amadori adducts. These polypeptides did not react with enzyme conjugate of carbohydrate-free albumin, confirming their binding specificity for glucose modified albumin and absence of co-identity with previously described albumin-binding proteins. The molecular weights of the glycated albumin binding polypeptides are distinct from those described for bovine albumin modified by advanced glycation end products or by formaldehyde, indicating that Amadori adducts in glycated albumin are recognized by unique endothelial cell binding sites.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0006-291X
pubmed:author
pubmed:issnType
Print
pubmed:day
30
pubmed:volume
193
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1131-6
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Identification of aortic endothelial cell binding proteins for Amadori adducts in glycated albumin.
pubmed:affiliation
University City Science Center, Philadelphia, PA 19104.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.