Linked Life Data

8319680

Source:http://linkedlifedata.com/resource/pubmed/id/8319680

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1993-8-3
pubmed:abstractText
The lipopolysaccharide, and particularly its lipid A moiety, of the J-5 mutant of Escherichia coli O111 plays a central role in studies on potential induction of cross-reactive and cross-protective antibodies, however, its chemical and antigenic structure was hitherto unknown. Here, the chemical structure of the J-5 lipid A is reported. It is composed of the bisphosphorylated disaccharide beta-D-GlcpN-4-P-(1-6)-alpha-D-GlcpN-1-P which carries four residues of 3-hydroxytetradecanoic acid, one each at positions 2, 3, 2', and 3'. The hydroxyl groups of the acyl residues at 2' and 3' are esterified with dodecanoic and tetradecanoic acid, respectively. The hydroxyl group at C-6' functions in the lipopolysaccharide as the attachment site of the core oligosaccharide. Furthermore, a new method to isolate the hydrophilic backbone, i.e. the 1,4'-bisphosphorylated glucosamine disaccharide, and its structural analysis by 1H-, 13C-, and 31P-NMR spectroscopy, are described, leading to a new and easier strategy in structural analysis of lipid A from bacterial lipopolysaccharides.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0014-2956
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
214
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
695-701
pubmed:dateRevised
2007-7-23
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Chemical structure of the lipid A of Escherichia coli J-5.
pubmed:affiliation
Division of Biochemical Microbiology, Forschungsinstitut Borstel, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't