Linked Life Data

8318225

Source:http://linkedlifedata.com/resource/pubmed/id/8318225

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1993-8-5
pubmed:abstractText
The plasma (PM), outer acrosomal (OAM), and inner acrosomal membranes (IAM) were isolated from rabbit and bull spermatozoa and the major phospholipids characterized. Choline-containing phospholipids, phosphatidylcholine (PC) and sphingomyelin (SM), constituted more than 60% of the total phospholipids (TPL) in all membranes of both species. Approximately more than 50% of PC in membrane preparations contained some form of ether linkage. Compared to OAM and IAM, cholesterol to phospholipid molar ratio was highest in PM of both species. Contrarily, protein to phospholipid ratio for PM was lowest compared to other membranes. The sphingomyelin to phosphatidylcholine ratio increased in the direction from PM to OAM to IAM. The hydrophobic fluorescent probe 1,6-diphenyl-1,3,5-hexatriene (DPH) was used to examine both the steady-state fluorescence anisotropy parameters and structural order parameter SDPH. The data showed higher rigidity in rabbit spermatozoa compared to bull spermatozoa (SDPH = 0.7582 and SDPH = 0.7326). In both species OAM had higher rigidity compared to the other two membranes (SDPH(OAM) = 0.7809, SDPH(PM) = 0.7308, and SDPH(IAM) = 0.7481 for bull; SDPH(OAM) = 0.8091, SDPH(PM) = 0.7857, and SDPH(IAM) = 0.7663 for rabbit). The inner leaflets of bull and rabbit spermatozoal membranes had significantly higher rigidity than the outer leaflets (for inner leaflet: rabbit-SDPH(PM) = 0.8391, SDPH(OAM) = 0.8149, and SDPH(IAM) = 0.7675; bull-SDPH(PM) = 0.8000, SDPH(OAM) = 0.7990, and SDPH(IAM) = 0.7990, and for outer leaflet: rabbit-SDPH(PM) = 0.7021, SDPH(OAM) = 0.7145, and SDPH(IAM) = 0.6867; bull-SDPH(PM) = 0.6986, SDPH(OAM) = 0.5980, and SDPH(IAM) = 0.7388).
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1040-452X
pubmed:author
pubmed:issnType
Print
pubmed:volume
35
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
209-17
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Phospholipids of rabbit and bull sperm membranes: structural order parameter and steady-state fluorescence anisotropy of membranes and membrane leaflets.
pubmed:affiliation
Department of Biochemistry, University of Georgia, Athens 30602.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.