Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1993-7-27
pubmed:abstractText
The possibility of using isozyme and PCR markers for estimation of preservation of human chromosomes in human.rodent somatic cell hybrids is considered. Methods of electrophoretic separation of 33 isozymes and 11 PCR markers for 22 human autosomes and X chromosome are described. Using these isozymes and PCR primers as markers of known regions and arms of human chromosomes, one can avoid errors in typization of chromosomes with complicated rearrangements which simulate similar patterns of G-banding. The method proposed in the paper not only facilitates considerably the analysis of hybrid clones, but is also an easy and reliable tool in selection of somatic cell hybrid clones for further investigation.
pubmed:language
rus
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0026-8984
pubmed:author
pubmed:issnType
Print
pubmed:volume
27
pubmed:geneSymbol
APOC2, C9, IFNA, IGLC2, PRIP, TG, TP53
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
685-707
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:articleTitle
[Use of isoenzyme and PCR markers for analyzing human chromosomes in human-rodent hybrid cells].
pubmed:publicationType
Journal Article, English Abstract