Linked Life Data

8316050

Source:http://linkedlifedata.com/resource/pubmed/id/8316050

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1993-7-23
pubmed:abstractText
The effects of increasing concentrations of eicosapentaenoic acid (20:5n-3; EPA) and oleic acid (18:1n-9; OA) on esterification to triacylglycerols (TG) and phospholipids (PL), and the relationship to formation and secretion of the very low density lipoproteins (VLDL) were compared in the isolated perfused rat liver. Mixtures of EPA and OA were also studied to determine whether substrate levels of one fatty acid might influence the metabolism of the other. The basal perfusion medium, which contained 30% (vol/vol) washed bovine erythrocytes, 6% (wt/vol) bovine serum albumin (BSA), and 100 mg glucose/dL in Krebs-Henseleit bicarbonate buffer (pH 7.4) was recycled through the liver for 2 h. EPA or OA, as a complex with 6% BSA, was infused at rates of 70, 105, 140 and 210 mumol/h. In other experiments, mixtures of EPA and oleic acid (70 mumol total), with molar percentages of 100, 75, 50, 25 and 0% of each fatty acid were infused per hour. BSA (6%) in the buffer was infused alone and served as the control. At an infusion rate of 70 mumol EPA per hour, hepatic VLDL lipid output was not different from that when fatty acid was not infused (approximately half that when 70 mumol OA/h was infused). However, when larger amounts of EPA and OA were infused individually, rates of VLDL secretion were stimulated to a similar extent with either fatty acid. The apparent inhibitory influence of EPA on TG synthesis and VLDL lipid output when 70 mumol EPA were infused per hour could also be overcome by the presence of as little as 25 mol% OA in a mixture.(ABSTRACT TRUNCATED AT 250 WORDS)
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0024-4201
pubmed:author
pubmed:issnType
Print
pubmed:volume
28
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
419-25
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Concentration-dependent effects of eicosapentaenoic acid on very low density lipoprotein secretion by the isolated perfused rat liver.
pubmed:affiliation
Department of Pharmacology, University of Tennessee, Memphis 38163.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.