Linked Life Data

8315861

Source:http://linkedlifedata.com/resource/pubmed/id/8315861

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1993-7-23
pubmed:abstractText
The DNA typing of HLA-DQA1 from bloodstains is described. Although amplification of DNA from bloodstains was sometimes difficult, the addition of BSA to the PCR medium induced good amplification. When a very limited amount (2 mm2, equivalent to 0.5 microliter blood) of bloodstain was analysed, the amplification was difficult. However, the addition of salmon sperm DNA to the DNA isolation medium, in the amount of 1 microgram per tube, yielded sufficient PCR products even when such a small amount of extract was analysed. DNA from bloodstains aged up to one year could be sufficiently amplified, if salmon sperm DNA was added to the DNA isolation medium, and BSA to the PCR medium.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0047-1887
pubmed:author
pubmed:issnType
Print
pubmed:volume
47
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6-12
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Amplification of HLA-DQA1 gene from bloodstains by polymerase chain reaction.
pubmed:affiliation
Department of Legal Medicine, Nagoya University School of Medicine, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't