Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1993-7-28
pubmed:abstractText
Two oligonucleotide primers were used in a polymerase chain reaction (PCR) procedure to amplify a region of the invasive-associated locus (ial) of Shigella and enteroinvasive Escherichia coli (EIEC). Detection of the amplified product can be done by agarose gel electrophoresis, which is specific and sensitive enough for routine diagnosis of these two pathogens. PCR is done using DNA extracted directly from faeces. The procedure can be completed in 7 h. These findings demonstrate a novel method for rapid, sensitive, specific, and simple diagnosis of diarrhoea caused by Shigella and EIEC.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0253-8768
pubmed:author
pubmed:issnType
Print
pubmed:volume
11
pubmed:geneSymbol
ial
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
38-40
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Detection of Shigella and enteroinvasive Escherichia coli using polymerase chain reaction.
pubmed:affiliation
East Hospital, Fujian, Peoples' Republic of China.
pubmed:publicationType
Journal Article