Linked Life Data

8314941

Source:http://linkedlifedata.com/resource/pubmed/id/8314941

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1993-7-27
pubmed:abstractText
An automated, internal standard high-performance liquid chromatographic method for the simultaneous quantitation of felbamate and its three metabolites in adult and neonatal rat brain and heart tissue homogenates was developed and validated. The homogenates prepared from one part of the tissue and four parts of water were extracted with ethyl acetate, and the extract was evaporated to dryness and redissolved in mobile phase. Separation was accomplished on a Waters Resolve C18, 5 microns, 300 mm x 3.9 mm I.D. column with a mobile phase consisting of 0.01 M phosphate buffer, pH 6.8-acetonitrile-methanol (800:150:50, v/v/v). Eluting peaks were monitored with an ultraviolet detector at 210 nm. The linear range of the assay for felbamate and the metabolites was 0.20-50.00 micrograms/ml of homogenate or 1-250 micrograms/g of brain or heart tissue. The lower limit of quantitation for all four analytes was 0.20 micrograms/ml of homogenate or 1.00 micrograms/g of tissue.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0021-9673
pubmed:author
pubmed:issnType
Print
pubmed:day
5
pubmed:volume
614
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
285-92
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Determination of the anticonvulsant felbamate and its three metabolites in brain and heart tissue of rats.
pubmed:affiliation
Department of Biochemistry, Wallace Laboratories, Division of Carter-wallace, Inc., Cranbury, NJ 08512.
pubmed:publicationType
Journal Article