Linked Life Data

8310423

Source:http://linkedlifedata.com/resource/pubmed/id/8310423

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1994-3-17
pubmed:abstractText
Repeated daily cocaine injections have been shown to alter mu-opioid receptor densities in the caudate putamen and nucleus accumbens of rat brain (Unterwald et al., 1991, 1992). Adenylyl cyclase activity was measured in rat rostral caudate putamen and nucleus accumbens following repeated cocaine administration to determine the functional consequences of cocaine-induced opioid receptor changes. Male Fischer rats were injected daily for 14 days with saline or cocaine HCl (30 or 45 mg/kg/day, i.p.) in three equal doses at 1-hr intervals. Basal adenylyl cyclase activity and the effects of the selective mu- and delta-opioid agonists [D-Ala2,N-Me-Phe4,Gly-ol5]enkephalin (DAMGO) and [D-penicillamine2,D-Penicillamine5]enkephalin (DPDPE), respectively, on adenylyl cyclase activity were examined 30 min after the last injection using a cAMP radioligand binding assay in crude membrane preparations. Basal adenylyl cyclase activity was 49% and 34% lower in the caudate putamen of animals treated with 30 and 45 mg/kg/day of cocaine, respectively, as compared to those receiving saline injections. Basal adenylyl cyclase activity was unchanged in the nucleus accumbens following cocaine treatment. DAMGO and DPDPE each maximally inhibited approximately 25% and 30%, respectively, of basal adenylyl cyclase in the caudate putamen and nucleus accumbens of saline-injected animals. Administration of cocaine attenuated the ability of DPDPE to inhibit adenylyl cyclase in both brain regions, but had no effect on the efficacy or potency of DAMGO for inhibiting adenylyl cyclase activity. These results suggest that chronic, repeated cocaine administration results in a selective impairment of delta-opioid receptor-mediated effector function in the caudate putamen and nucleus accumbens.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0887-4476
pubmed:author
pubmed:issnType
Print
pubmed:volume
15
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
33-8
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:8310423-Adenylate Cyclase, pubmed-meshheading:8310423-Analgesics, pubmed-meshheading:8310423-Animals, pubmed-meshheading:8310423-Caudate Nucleus, pubmed-meshheading:8310423-Cocaine, pubmed-meshheading:8310423-Cyclic AMP, pubmed-meshheading:8310423-Drug Administration Schedule, pubmed-meshheading:8310423-Enkephalin, Ala(2)-MePhe(4)-Gly(5)-, pubmed-meshheading:8310423-Enkephalin, D-Penicillamine (2,5)-, pubmed-meshheading:8310423-Enkephalins, pubmed-meshheading:8310423-Injections, Intraperitoneal, pubmed-meshheading:8310423-Male, pubmed-meshheading:8310423-Nucleus Accumbens, pubmed-meshheading:8310423-Putamen, pubmed-meshheading:8310423-Rats, pubmed-meshheading:8310423-Rats, Inbred F344, pubmed-meshheading:8310423-Receptors, Opioid, delta, pubmed-meshheading:8310423-Receptors, Opioid, mu, pubmed-meshheading:8310423-Reference Values
pubmed:year
1993
pubmed:articleTitle
Chronic repeated cocaine administration alters basal and opioid-regulated adenylyl cyclase activity.
pubmed:affiliation
Rockefeller University, New York, New York 10021.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.