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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1994-3-15
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pubmed:abstractText |
Mammalian cell lines were transfected with antibody heavy (H) chain-ricin A chain gene fusions in attempts to assemble a recombinant immunotoxin. We found that a light chain-secreting mouse plasmacytoma cell line can be transfected stably with such a chimaeric gene, but only if the ricin A chain portion is disarmed by genetic means prior to transfection; if not, stable transfection appears to select for genetic inactivation of the transfected gene. Co-expression of an antibody heavy chain-ricin A chain fusion with light chain in non-lymphoid cells results in cell death. We conclude that the ricin A chain moiety retains biological activity precluding the expression of biologically active antibody-ricin A chain fusion proteins in mammalian cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0161-5890
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
31
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
117-25
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:8309475-Amino Acid Sequence,
pubmed-meshheading:8309475-Animals,
pubmed-meshheading:8309475-Base Sequence,
pubmed-meshheading:8309475-Blotting, Southern,
pubmed-meshheading:8309475-Cell Line,
pubmed-meshheading:8309475-Cloning, Molecular,
pubmed-meshheading:8309475-DNA,
pubmed-meshheading:8309475-Immunoglobulin Heavy Chains,
pubmed-meshheading:8309475-Immunotoxins,
pubmed-meshheading:8309475-Mammals,
pubmed-meshheading:8309475-Mice,
pubmed-meshheading:8309475-Mice, Inbred C57BL,
pubmed-meshheading:8309475-Molecular Sequence Data,
pubmed-meshheading:8309475-Plasmids,
pubmed-meshheading:8309475-Polymerase Chain Reaction,
pubmed-meshheading:8309475-Recombinant Proteins,
pubmed-meshheading:8309475-Ricin,
pubmed-meshheading:8309475-Transfection,
pubmed-meshheading:8309475-Tumor Cells, Cultured
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pubmed:year |
1994
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pubmed:articleTitle |
Expression of immunoglobulin heavy chain-ricin A chain fusions in mammalian cells.
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pubmed:affiliation |
Department of Clinical Oncology, Royal Postgraduate Medical School, Hammersmith Hospital, London, U.K.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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