Linked Life Data

8308021

Source:http://linkedlifedata.com/resource/pubmed/id/8308021

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1994-3-17
pubmed:databankReference
pubmed:abstractText
An intronless gene encoding a protein of 652 amino acid residues with an M(r) of 71,266, showing between 79% and 59% identity in nucleotide sequence with heat shock protein 70 (HSP 70) genes of Bremia lactucae (a parasitic Oomycete of lettuce) and a wide range of organisms that include humans, was isolated from the nonparasitic Oomycete Achlya klebsiana. While the gene appears to be constitutively expressed, L-glutamine augmented its expression particularly under conditions of nutritional stress. L-Glutamine enhanced the transcription of a 2.4-kilobase poly(A)+ RNA simultaneously in the same way as it elevated the cellular level of the HSP 70-like protein. A polyclonal antibody (affinity-purified) raised in rabbit against the purified monomeric (M(r) 120,000) form of an NAD-specific glutamate dehydrogenase (Yang, B., and LéJohn, H.B. (1994) J. Biol. Chem. 269, 4506-4512) immunoprecipitated the HSP 70-like protein, and it was used to study the kinetics of induction of this stress-related protein and the effect of proteinase inhibitors on its metabolism. By using as probes four partial length cDNA clones, nine overlapping DNA fragments of the organism's genome carrying the HSP 70-like protein gene were isolated from a genomic library. The nucleotide sequence of the gene, including its boundaries, was determined by using these genomic clones. The 5'-untranslated boundary of the gene displayed the classical nucleotide arrangement of heat shock elements as well as CCAAT and TATA box motifs. Within the coding region are the typical conserved amino acid heat shock protein signatures 1 and 2 at the predicted locations. By primer extension and S1 nuclease protection mapping system, we estimated that the gene is probably transcribed into a message of 2.2 kilobases.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
11
pubmed:volume
269
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4513-22
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:8308021-Amino Acid Sequence, pubmed-meshheading:8308021-Base Sequence, pubmed-meshheading:8308021-Cloning, Molecular, pubmed-meshheading:8308021-DNA, Complementary, pubmed-meshheading:8308021-DNA, Fungal, pubmed-meshheading:8308021-DNA Primers, pubmed-meshheading:8308021-Gene Expression Regulation, Fungal, pubmed-meshheading:8308021-Genes, Fungal, pubmed-meshheading:8308021-Glucose, pubmed-meshheading:8308021-Glutamate Dehydrogenase, pubmed-meshheading:8308021-Glutamine, pubmed-meshheading:8308021-Heat-Shock Proteins, pubmed-meshheading:8308021-Molecular Sequence Data, pubmed-meshheading:8308021-Nitrogen, pubmed-meshheading:8308021-Oomycetes, pubmed-meshheading:8308021-RNA, Fungal, pubmed-meshheading:8308021-RNA, Messenger, pubmed-meshheading:8308021-Restriction Mapping, pubmed-meshheading:8308021-Sequence Alignment, pubmed-meshheading:8308021-Sequence Homology, Amino Acid, pubmed-meshheading:8308021-Sequence Homology, Nucleic Acid, pubmed-meshheading:8308021-Solubility
pubmed:year
1994
pubmed:articleTitle
Cloning and analysis of a constitutive heat shock (cognate) protein 70 gene inducible by L-glutamine.
pubmed:affiliation
Department of Microbiology, University of Manitoba, Winnipeg, Canada.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't