Linked Life Data

8306712

Source:http://linkedlifedata.com/resource/pubmed/id/8306712

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
1994-3-14
pubmed:abstractText
We assigned the gene for tear lipocalin to the long arm of human chromosome 9. Polyadenylated RNA was extracted from lacrimal gland. The coding region for tear lipocalin was amplified, sequenced and used to probe a panel of somatic cell hybrid DNA by Southern blot analysis. Regional mapping was accomplished by probing a panel of subfragments of the indicated chromosome. Restriction of genomic DNA with EcoRI failed to reveal any bands corresponding to the human tear lipocalin gene in mouse-human hybrids all of which lack chromosome 9. Southern blot analysis of human-hamster hybrids demonstrated a human 5.6 kb TaqI restriction fragment that segregated to the q34-qter region of chromosome 9 and assigned the gene for tear lipocalin to this region. Structurally homologous proteins of the lipocalin family, human placental protein 14, human alpha 1 microglobulin, and human brain prostaglandin synthase, have been mapped to this region. We suggest that the gene for tear lipocalin is part of an important lipocalin superfamily gene cluster on chromosome 9 within band q34.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0271-3683
pubmed:author
pubmed:issnType
Print
pubmed:volume
12
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1019-23
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Assignment of tear lipocalin gene to human chromosome 9q34-9qter.
pubmed:affiliation
Department of Ophthalmology, Jules Stein Eye Institute, UCLA School of Medicine 90024.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't