Linked Life Data

8306467

Source:http://linkedlifedata.com/resource/pubmed/id/8306467

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
1994-3-17
pubmed:abstractText
The effects of choice of solvent and period of extraction on recovery of phospholipid classes extracted from rat erythrocyte membranes were investigated. For extraction of phosphatidylserine (PS) the best recovery was achieved using chloroform-methanol (2:1, v/v), for diacyl phosphatidylethanolamine (PE) acetone-isopropanol (3:2, v/v), and for extraction of plasmalogen PE, phosphatidylcholine (PC) and sphingomyelin (SM) using hexane-isopropanol (3:2, v/v). Using hexane-isopropanol (3:2, v/v), boiling during the extraction increased the recovery of some of the individual phospholipid classes whereas mechanical treatment of the sample had only a slight effect. Furthermore, the fatty acid composition of PS and PE varied most with different extraction conditions and the phospholipid classes PC and SM were extracted more readily from rat erythrocyte membranes than PS, diacyl and plasmalogen PE.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0009-8981
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
219
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
93-104
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Studies on the extraction of phospholipids from erythrocyte membranes in the rat.
pubmed:affiliation
Institut für Ernährungsphysiologie, Technischen Universität München-Weihenstephan, Freising, Germany.
pubmed:publicationType
Journal Article