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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1994-3-16
|
| pubmed:abstractText |
Northern blot hybridization, reverse-transcription polymerase chain reaction (RT-PCR), and RNase protection assays were used to examine the expression of two alpha 1(IX) collagen mRNA species (long and short form) in developing mouse tissues. Furthermore, in situ hybridization was used to identify cells expressing the Col9a1 gene during eye development. The results indicate that during embryonic development eye and heart preferentially express the short form; lung and cartilage express the long form; whereas liver expresses a very low level of long form alpha 1(IX) mRNA which can only be detected by RT-PCR. In situ hybridization demonstrated that at 10.5 day postcoitum (d.p.c.), the alpha 1(IX) collagen mRNAs were first expressed in optic cup (neural ectoderm) but not in lens vesicle (surface ectoderm). By 13.5 d.p.c., the cells that express the alpha 1(IX) mRNA progressively were concentrated toward the anterior part of the neural retina. By 16.5-18.5 d.p.c., the hybridization signals were found exclusively in the inner non-pigmented layer of the presumptive ciliary epithelium. As ciliary epithelial cells become well differentiated 3 weeks after birth, cells expressing the Col9a1 gene were limited to the junction between mature ciliary folds and the neural retina. No hybridization signal could be detected in ocular tissues of mouse older than 6 weeks. It is of interest to note that a hybridization signal was not detected in cornea at the various developmental stages examined, suggesting that mouse cornea does not significantly express alpha 1(IX) mRNA during embryonic development. This differs from that of chick cornea development. In summary, the expression of the Col9a1 gene shows a temporospatial pattern throughout mouse eye development.(ABSTRACT TRUNCATED AT 250 WORDS)
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
1058-8388
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
198
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
150-7
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| pubmed:dateRevised |
2007-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:8305707-Animals,
pubmed-meshheading:8305707-Base Sequence,
pubmed-meshheading:8305707-Blotting, Northern,
pubmed-meshheading:8305707-Blotting, Southern,
pubmed-meshheading:8305707-Collagen,
pubmed-meshheading:8305707-Cornea,
pubmed-meshheading:8305707-DNA,
pubmed-meshheading:8305707-Embryo, Mammalian,
pubmed-meshheading:8305707-Embryonic and Fetal Development,
pubmed-meshheading:8305707-Heart,
pubmed-meshheading:8305707-In Situ Hybridization,
pubmed-meshheading:8305707-Isomerism,
pubmed-meshheading:8305707-Liver,
pubmed-meshheading:8305707-Lung,
pubmed-meshheading:8305707-Mice,
pubmed-meshheading:8305707-Molecular Sequence Data,
pubmed-meshheading:8305707-Myocardium,
pubmed-meshheading:8305707-Polymerase Chain Reaction,
pubmed-meshheading:8305707-RNA, Messenger,
pubmed-meshheading:8305707-Retina
|
| pubmed:year |
1993
|
| pubmed:articleTitle |
Developmental patterns of two alpha 1(IX) collagen mRNA isoforms in mouse.
|
| pubmed:affiliation |
Department of Ophthalmology, University of Cincinnati, Ohio 45267-0527.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|