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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1 Pt 2
|
| pubmed:dateCreated |
1994-3-9
|
| pubmed:abstractText |
The effects of endotoxin on endothelial and smooth muscle function were investigated in small femoral arteries removed from rats 4 h after intraperitoneal injection of Escherichia coli lipopolysaccharide (LPS; 20 mg/kg) or solvent. In the absence of L-arginine in the organ bath, the sensitivity of the arteries to norepinephrine (NE) was decreased only slightly, and the relaxing effects of neither 3-morpholinosydonimine-N-ethyl-carbamide (SIN-1), a nitric oxide (NO) donor, nor acetylcholine (ACh) were modified by LPS treatment despite morphological damage to the endothelium seen with scanning electron microscopy. However, L-arginine (30 microM to 1 mM), which had no effect on control vessels, caused a rapid and stereospecific relaxation of arteries from LPS-treated rats that was abolished by both NG-nitro-L-arginine methyl ester (1 mM), a NO synthase inhibitor, and methylene blue, an inhibitor of the activation of guanylyl cyclase by NO. The relaxing effect of L-arginine was observed in the absence of endothelium, although it was significantly greater in its presence. In addition, a 30-min exposure to extracellular L-arginine (100 microM) moderately but significantly decreased the sensitivity to ACh and SIN-1 of vessels from LPS-treated but not from control rats. These results indicate that LPS treatment induced a NO synthase activity in smooth muscle cells of rat small femoral arteries and that the resulting relaxation was dependent on extracellular L-arginine in these resistance vessels.(ABSTRACT TRUNCATED AT 250 WORDS)
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
|
| pubmed:issn |
0002-9513
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
266
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
H191-8
|
| pubmed:dateRevised |
2003-11-14
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| pubmed:meshHeading |
pubmed-meshheading:8304499-Animals,
pubmed-meshheading:8304499-Arginine,
pubmed-meshheading:8304499-Endothelium, Vascular,
pubmed-meshheading:8304499-Escherichia coli,
pubmed-meshheading:8304499-Femoral Artery,
pubmed-meshheading:8304499-Lipopolysaccharides,
pubmed-meshheading:8304499-Male,
pubmed-meshheading:8304499-Microscopy, Electron, Scanning,
pubmed-meshheading:8304499-Muscle, Smooth, Vascular,
pubmed-meshheading:8304499-Rats,
pubmed-meshheading:8304499-Rats, Wistar,
pubmed-meshheading:8304499-Vasoconstriction
|
| pubmed:year |
1994
|
| pubmed:articleTitle |
Effect of bacterial lipopolysaccharide on function of rat small femoral arteries.
|
| pubmed:affiliation |
Laboratoire de Pharmacologie Cellulaire et Moléculaire, Université Louis Pasteur de Strasbourg, Illkirch, France.
|
| pubmed:publicationType |
Journal Article
|