Linked Life Data

8304050

Source:http://linkedlifedata.com/resource/pubmed/id/8304050

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
1994-3-8
pubmed:abstractText
Although analogs and metabolites of vitamin D have been tested for their calciotropic activity, very little information has been available concerning the effects of these compounds on gene expression. In this study one analog of vitamin D, 1,25,28-trihydroxyvitamin D2 [1,25,28-(OH)3D2], and one metabolite, 1,24,25-trihydroxyvitamin D3 [1,24,25-(OH)3D3], were tested for their effect on intestinal calbindin-D9K mRNA and protein as well as for their effect on intestinal calcium absorption and bone calcium mobilization. These compounds were also evaluated for their ability to compete for rat intestinal 1,25-(OH)2D3 receptor sites and to induce differentiation of human leukemia (HL-60) cells as indicated by reduction of nitro blue tetrazolium. In vivo studies involved intrajugular injection of 12.5 ng 1,25-(OH)2D3 or test compound to vitamin D-deficient rats and sacrifice after 18 h. 1,25,28-Trihydroxyvitamin D2 had no effect on intestinal calcium absorption, bone calcium mobilization, or intestinal calbindin-D9K protein and mRNA. Competitive binding to 1,25-(OH)2D3 receptors was 0.8% of that observed using 1,25-(OH)2D3. However, 20- and 40-fold higher doses of 1,25,28-(OH)3D2 (250 and 500 ng) resulted in significant inductions in calbindin-D9K protein and mRNA (3.5 to 7.4-fold), although doses as high as 800 ng were found to have no effect on intestinal calcium absorption or bone calcium mobilization.(ABSTRACT TRUNCATED AT 250 WORDS)
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0884-0431
pubmed:author
pubmed:issnType
Print
pubmed:volume
8
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1483-90
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:8304050-25-Hydroxyvitamin D 2, pubmed-meshheading:8304050-Animals, pubmed-meshheading:8304050-Binding, Competitive, pubmed-meshheading:8304050-Biological Transport, pubmed-meshheading:8304050-Bone and Bones, pubmed-meshheading:8304050-Calcitriol, pubmed-meshheading:8304050-Calcium, pubmed-meshheading:8304050-Calcium-Binding Protein, Vitamin D-Dependent, pubmed-meshheading:8304050-Cell Differentiation, pubmed-meshheading:8304050-Dose-Response Relationship, Drug, pubmed-meshheading:8304050-Duodenum, pubmed-meshheading:8304050-Humans, pubmed-meshheading:8304050-Hydroxycholecalciferols, pubmed-meshheading:8304050-Intestinal Absorption, pubmed-meshheading:8304050-Male, pubmed-meshheading:8304050-RNA, Messenger, pubmed-meshheading:8304050-Rats, pubmed-meshheading:8304050-Receptors, Calcitriol, pubmed-meshheading:8304050-Tumor Cells, Cultured, pubmed-meshheading:8304050-Vitamin D Deficiency
pubmed:year
1993
pubmed:articleTitle
Effect of 1,25,28-trihydroxyvitamin D2 and 1,24,25-trihydroxyvitamin D3 on intestinal calbindin-D9K mRNA and protein: is there a correlation with intestinal calcium transport?
pubmed:affiliation
Department of Biochemistry and Molecular Biology, University of Medicine and Dentistry of New Jersey Medical School and Graduate School of Biomedical Sciences, Newark.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.