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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1994-3-10
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pubmed:databankReference | |
pubmed:abstractText |
Serotonin (5-hydroxytryptamine or 5-HT) is an important biogenic amine that functions as both a neurotransmitter and a hormone in the central nervous system (CNS) and the periphery. We report here the isolation of a cDNA from the OK cell that encodes a serotonin receptor (OKc1). When expressed in cultured cells, it displayed the pharmacological profile and negative coupling with adenylyl cyclase characteristic of a 5-HT1B receptor subtype. Similar to the cloned rodent 5-HT1B receptors, it had high affinity for the beta-adrenergic ligand [125I]iodocyanopindolol, because of the presence of an asparagine instead of a threonine residue in the seventh transmembrane region. The ligands used displayed the following rank order of potencies: cyanopindolol > RU24969 > methiothepin > serotonin > sumatriptan > methysergide > 8-OH-DPAT > isoproterenol. This profile correlates well (r = 0.97) with the native OK cell 5-HT1B receptor. When OKc1 is compared to the rat, mouse, and human 5-HT1B receptors, it has an amino acid sequence identity of 82%, but it is only 54% identical to the human 5-HT1D receptor.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0026-895X
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
45
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
20-8
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:8302276-Amino Acid Sequence,
pubmed-meshheading:8302276-Animals,
pubmed-meshheading:8302276-Base Sequence,
pubmed-meshheading:8302276-CHO Cells,
pubmed-meshheading:8302276-Cells, Cultured,
pubmed-meshheading:8302276-Cloning, Molecular,
pubmed-meshheading:8302276-Cricetinae,
pubmed-meshheading:8302276-DNA, Complementary,
pubmed-meshheading:8302276-Kidney,
pubmed-meshheading:8302276-Ligands,
pubmed-meshheading:8302276-Mice,
pubmed-meshheading:8302276-Molecular Sequence Data,
pubmed-meshheading:8302276-Opossums,
pubmed-meshheading:8302276-Rats,
pubmed-meshheading:8302276-Receptors, Serotonin,
pubmed-meshheading:8302276-Sequence Homology, Amino Acid
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pubmed:year |
1994
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pubmed:articleTitle |
The cloning and expression of an OK cell cDNA encoding a 5-hydroxytryptamine1B receptor.
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pubmed:affiliation |
Department of Pharmacology, University of Nebraska Medical Center, Omaha 68198-6260.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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