Linked Life Data

8301146

Source:http://linkedlifedata.com/resource/pubmed/id/8301146

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1994-3-9
pubmed:databankReference
pubmed:abstractText
The production of pathogenic anti-DNA autoantibodies in mice with lupus nephritis is dependent on special autoimmune Th cells that can also transfer the disease into preautoimmune mice. In previous work, these pathogenic Th cells were cloned and their TCR beta-chains were sequenced to reveal a recurrent motif of anionic residues in their CDR3 loops. Accordingly, approximately half of the Th clones were found to be specific for nucleosomal Ag that contain cationic residues. Herein, we analyzed the TCR alpha-chain repertoire of 15 of these pathogenic Th clones and found them to be heterogeneous, even among the nucleosome-specific Th clones. Most of these autoimmune TCR alpha-chains contained anionic residues in their CDR3 in addition to cationic residues. Therefore, these pathogenic Th clones of lupus probably recognize epitopes with mixed charge runs that are derived from autoantigens, such as histone-DNA complexes. Interestingly, the V alpha gene segments used by 10 of these Th clones derived from the (SWR x NZB)F1 lupus mice differed from previously reported sequences indicating that they were new members or alleles of the respective V alpha gene family. One of the Th clones used a gene from an entirely new murine V alpha gene family, identified here as V alpha 23, which consisted of approximately two members that were conserved among strains with different V alpha haplotypes. Knowledge of the primary structure of the TCR expressed by these pathogenic Th clones of lupus would help in the analysis of their antigenic specificities and also would be essential for studying their regulation in transgenic mice carrying these autoimmune TCR genes.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
152
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1462-70
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:8301146-Amino Acid Sequence, pubmed-meshheading:8301146-Animals, pubmed-meshheading:8301146-Autoantibodies, pubmed-meshheading:8301146-Base Sequence, pubmed-meshheading:8301146-DNA Primers, pubmed-meshheading:8301146-Gene Rearrangement, alpha-Chain T-Cell Antigen Receptor, pubmed-meshheading:8301146-Genes, pubmed-meshheading:8301146-Lupus Erythematosus, Systemic, pubmed-meshheading:8301146-Mice, pubmed-meshheading:8301146-Molecular Sequence Data, pubmed-meshheading:8301146-Polymorphism, Genetic, pubmed-meshheading:8301146-Receptors, Antigen, T-Cell, alpha-beta, pubmed-meshheading:8301146-Sequence Alignment, pubmed-meshheading:8301146-Sequence Homology, Amino Acid, pubmed-meshheading:8301146-Sequence Homology, Nucleic Acid, pubmed-meshheading:8301146-T-Lymphocytes, pubmed-meshheading:8301146-T-Lymphocytes, Helper-Inducer
pubmed:year
1994
pubmed:articleTitle
T cell receptor alpha-chain repertoire of pathogenic autoantibody-inducing T cells in lupus mice.
pubmed:affiliation
Department of Medicine (Arthritis Division), Northwestern University Medical School, Chicago, IL 60611.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.