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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1994-3-4
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pubmed:abstractText |
The mechanism of resistance to human complement-mediated killing in Moraxella catarrhalis was studied by comparing different complement-sensitive and complement-resistant M. catarrhalis strains in a functional bystander hemolysis assay and an enzyme-linked immunosorbent assay (ELISA) for soluble terminal complement complexes. Complement-resistant stains appeared to activate complement to the same extent as, or even slightly better than, complement-sensitive strains. This indicates that complement-resistant strains do not inhibit classical or alternative pathway activation but interfere with complement at the level of membrane attack complex formation. A clear difference in dose-response curves for resistant and sensitive strains was observed both in the bystander hemolysis assay and in the ELISA. Complement-resistant strains showed optimum curves, whereas complement-sensitive strains gave almost linear curves. We conclude that resistant strains bind and/or inactivate one of the terminal complement components or intermediates involved in membrane attack complex formation. Trypsin, known to abolish complement resistance, changed the optimum dose-response curve of a resistant strain to a linear one, which strongly suggests that complement resistance is mediated by an M. catarrhalis-associated protein. This protein acts directly or through the binding of a terminal complement inhibitor present in serum.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8300216-1372874,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8300216-14106311,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8300216-1708379,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8300216-1725600,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8300216-1905385,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8300216-1934605,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8300216-2121328,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8300216-2167295,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8300216-2412280,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8300216-2454950,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8300216-2471750,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8300216-2501353,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8300216-3122301,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8300216-3918112,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8300216-3921623,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8300216-4035298,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8300216-6715891,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8300216-7430645,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8300216-7682159,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8300216-8098421,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8300216-823639,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8300216-8442864
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0019-9567
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
62
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
589-95
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1994
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pubmed:articleTitle |
Differences in complement activation between complement-resistant and complement-sensitive Moraxella (Branhamella) catarrhalis strains occur at the level of membrane attack complex formation.
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pubmed:affiliation |
Eijkman-Winkler Institute of Medical and Clinical Microbiology, Utrecht University Medical Faculty, University Hospital, The Netherlands.
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pubmed:publicationType |
Journal Article,
Comparative Study,
In Vitro
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