Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1994-3-10
|
| pubmed:abstractText |
The growth-promoting activities of interleukin-10 (IL-10) were assessed in hematopoietic colony-forming assays. We found that IL-10 failed to support the clonal growth of normal and lineage-depleted (Lin-) bone marrow (BM) cells. Furthermore, IL-10 neither enhanced nor suppressed colony formation by eosinophil, neutrophil, or macrophage progenitors when combined with a variety of factors. IL-10 stimulated a modest increase in erythropoietin (Epo)-dependent erythroid colonies but had no effect on the burst-promoting activities of IL-3. However, the combination of IL-10 plus IL-3 resulted in the enhanced growth of mast cell progenitors. In addition to its mast cell stimulating activity, IL-10 promoted the growth of megakaryocyte (Mk) and Mk-mixed colonies when combined with Epo or with Epo plus IL-3, IL-6, or IL-11. Comparative studies showed that the megakaryocyte potentiating activity of IL-10 is roughly equivalent to that of IL-6 and IL-11. In experiments using Thy1loSca1+ stem cells, IL-10 was shown to enhance the number of cells initiating IL-3-dependent colony formation. IL-10 also costimulated increased colony formation when used with IL-3 and another factor such as IL-1, IL-6, and granulocyte colony-stimulating factor (G-CSF). Cellular analysis of the resulting colonies indicated that IL-10 increases the formation of multilineage colonies containing erythrocytes, megakaryocytes, and/or mast cells. The ability of IL-10 to cooperatively regulate various stages of hematopoietic development is discussed.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Colony-Stimulating Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Erythropoietin,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-10,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-3,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
0301-472X
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
22
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
136-41
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:8299735-Animals,
pubmed-meshheading:8299735-Cell Differentiation,
pubmed-meshheading:8299735-Cell Division,
pubmed-meshheading:8299735-Colony-Forming Units Assay,
pubmed-meshheading:8299735-Colony-Stimulating Factors,
pubmed-meshheading:8299735-Dose-Response Relationship, Drug,
pubmed-meshheading:8299735-Erythroid Precursor Cells,
pubmed-meshheading:8299735-Erythropoietin,
pubmed-meshheading:8299735-Granulocytes,
pubmed-meshheading:8299735-Hematopoietic Stem Cells,
pubmed-meshheading:8299735-Interleukin-10,
pubmed-meshheading:8299735-Interleukin-3,
pubmed-meshheading:8299735-Mast Cells,
pubmed-meshheading:8299735-Megakaryocytes,
pubmed-meshheading:8299735-Mice,
pubmed-meshheading:8299735-Mice, Inbred C57BL,
pubmed-meshheading:8299735-Mice, Inbred CBA,
pubmed-meshheading:8299735-Recombinant Proteins
|
| pubmed:year |
1994
|
| pubmed:articleTitle |
Interleukin-10 promotes the growth of megakaryocyte, mast cell, and multilineage colonies: analysis with committed progenitors and Thy1loSca1+ stem cells.
|
| pubmed:affiliation |
Department of Immunology, DNAX Research Institute, Palo Alto, CA 94304.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|