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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
1994-3-4
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pubmed:abstractText |
A method for the preparation of a new packing material for high performance liquid chromatography by bonding an anti-interferon monoclonal antibody to the surface of silica gel is described. The high coupling efficiency and activity of the interferon-alpha A-monoclonal antibody (IFN-alpha A-McAb) column were obtained by activated diol-silica gel with an activating agent. After purification with this packing material, the specific activity of recombinant human interferon-alpha A rose by up to 1.03 x 10(7)IU/mg protein and the purification efficiency was increased by approximately 100 times.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
0269-3879
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
7
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
317-20
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
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pubmed:articleTitle |
Studies on silica-bonded monoclonal antibody packing material for separation of recombinant interferon by high performance immunoaffinity chromatography.
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pubmed:affiliation |
Department of Chemistry, Northwest University, Xi'an, China.
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pubmed:publicationType |
Journal Article
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