Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5-6
|
| pubmed:dateCreated |
1994-3-10
|
| pubmed:abstractText |
The hematopoietic stimulating activity of a human lung cancer cell line, MC-1, was investigated. The protein fraction (MC-1 protein) was prepared from the serum-free culture supernatant of MC-1 cells using hydroxyapatite and concanavalin A-agarose columns. In serum-containing cultures, MC-1 protein stimulated colony formation by megakaryocyte colony-forming units (CFU), erythroid burst-forming units and granulocyte/macrophage (GM) CFU. The stimulating effect was strongest for megakaryocyte CFU. The factor having megakaryocyte colony-stimulating activity was shown to be a protein whose molecular weight was determined to be 23,000 daltons by gel filtration. By various analyses, this protein was shown to be molecularly different from the heretofore-identified cytokines that may affect megakaryocytopoiesis, i.e., interleukin-1 (IL-1), IL-2, IL-3, IL-6, IL-7, IL-11, granulocyte colony-stimulating factor (CSF), macrophage CSF, GM-CSF, leukemia inhibitory factor, stem cell factor and tumor necrosis factor. Under serum-free conditions, MC-1 protein augmented murine megakaryocyte colony formation in the presence of murine IL-3 and increased the acetylcholinesterase activity of purified murine megakaryocytes. It was also shown that MC-1 protein stimulated human megakaryocyte colony formation. It was concluded that MC-1 cells produce a megakaryocyte potentiator which is molecularly different from any heretofore-identified cytokines.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Culture Media,
http://linkedlifedata.com/resource/pubmed/chemical/Culture Media, Serum-Free,
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/Hematopoietic Cell Growth Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-3,
http://linkedlifedata.com/resource/pubmed/chemical/Neoplasm Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger
|
| pubmed:status |
MEDLINE
|
| pubmed:issn |
1015-2008
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
61
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
256-67
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:8297493-Animals,
pubmed-meshheading:8297493-Carcinoma, Non-Small-Cell Lung,
pubmed-meshheading:8297493-Cell Differentiation,
pubmed-meshheading:8297493-Cell Division,
pubmed-meshheading:8297493-Culture Media,
pubmed-meshheading:8297493-Culture Media, Serum-Free,
pubmed-meshheading:8297493-Cytokines,
pubmed-meshheading:8297493-Erythrocytes,
pubmed-meshheading:8297493-Granulocytes,
pubmed-meshheading:8297493-Hematopoiesis,
pubmed-meshheading:8297493-Hematopoietic Cell Growth Factors,
pubmed-meshheading:8297493-Humans,
pubmed-meshheading:8297493-Interleukin-3,
pubmed-meshheading:8297493-Lung Neoplasms,
pubmed-meshheading:8297493-Megakaryocytes,
pubmed-meshheading:8297493-Mice,
pubmed-meshheading:8297493-Mice, Inbred C57BL,
pubmed-meshheading:8297493-Neoplasm Proteins,
pubmed-meshheading:8297493-RNA, Messenger,
pubmed-meshheading:8297493-Tumor Cells, Cultured
|
| pubmed:year |
1993
|
| pubmed:articleTitle |
A novel megakaryocyte potentiator produced by MC-1 human lung cancer cell line.
|
| pubmed:affiliation |
Department of Medicine III, School of Medicine, Iwate Medical University, Morioka, Japan.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|