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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
1994-3-1
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pubmed:abstractText |
This study was undertaken to search for the endogenous dithiol cofactor of the reductases of the vitamin K cycle. As a starting point, the redox-active lipophilic endogenous compounds lipoic acid and lipoamide were looked at. The study shows that microsomes contain NADH-dependent lipoamide reductase activity. Reduced lipoamide stimulates microsomal vitamin K epoxide reduction with kinetics comparable with those for the synthetic dithiol dithiothreitol (DTT). Reduced lipoic acid shows higher (4-fold) Km values. No reductase activity with lipoic acid was found to be present in microsomes or cytosol. The reduced-lipoamide-stimulated vitamin K epoxide reductase is as sensitive to warfarin and salicylate inhibition as is the DTT-stimulated one. Both vitamin K epoxide reductase and lipoamide reductase activity are recovered in the rough microsomes. NADH/lipoamide-stimulated vitamin K epoxide reduction is uncoupled by traces of Triton X-100, suggesting that microsomal lipoamide reductase and vitamin K epoxide reductase are associated. The results suggest that the vitamin K cycle obtains reducing equivalents from NADH through microsomal lipoamide reductase.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8297331-1299627,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8297331-1731762,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8297331-1740407,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8297331-1958236,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8297331-2183788,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8297331-2188973,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8297331-2419338,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8297331-2668278,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8297331-3207986,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8297331-3308517,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8297331-3371540,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8297331-3549509,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8297331-3632704,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8297331-3896234,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8297331-6149281,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8297331-6652076,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8297331-6733086,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8297331-6771170,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8297331-6833262,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8297331-6896050,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8297331-8217828
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Dihydrolipoamide Dehydrogenase,
http://linkedlifedata.com/resource/pubmed/chemical/Dithiothreitol,
http://linkedlifedata.com/resource/pubmed/chemical/Mixed Function Oxygenases,
http://linkedlifedata.com/resource/pubmed/chemical/NADH, NADPH Oxidoreductases,
http://linkedlifedata.com/resource/pubmed/chemical/Salicylic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Salicylic Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Thioctic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Warfarin,
http://linkedlifedata.com/resource/pubmed/chemical/lipoamide,
http://linkedlifedata.com/resource/pubmed/chemical/vitamin K epoxidase
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
297 ( Pt 2)
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
277-80
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8297331-Animals,
pubmed-meshheading:8297331-Dihydrolipoamide Dehydrogenase,
pubmed-meshheading:8297331-Dithiothreitol,
pubmed-meshheading:8297331-Microsomes, Liver,
pubmed-meshheading:8297331-Mixed Function Oxygenases,
pubmed-meshheading:8297331-NADH, NADPH Oxidoreductases,
pubmed-meshheading:8297331-Rats,
pubmed-meshheading:8297331-Salicylic Acid,
pubmed-meshheading:8297331-Salicylic Acids,
pubmed-meshheading:8297331-Thioctic Acid,
pubmed-meshheading:8297331-Warfarin
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pubmed:year |
1994
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pubmed:articleTitle |
Microsomal lipoamide reductase provides vitamin K epoxide reductase with reducing equivalents.
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pubmed:affiliation |
Department of Pharmacology, University of Limburg, Maastricht, The Netherlands.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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