Linked Life Data

8294929

Source:http://linkedlifedata.com/resource/pubmed/id/8294929

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1994-3-2
pubmed:abstractText
Zinc-65 transport into different regions of rat brain has been measured during short vascular perfusion of one cerebral hemisphere with an oxygenated HEPES-containing physiological saline at pH 7.40. The [Zn2+] was buffered with either bovine serum albumin or histidine. In each case uptake was linear with time up to 90 s. 65Zn flux into brain in the presence of albumin followed Michaelis-Menten kinetics and for parietal cortex had a Km of 16 nM and a Vmax of 44 nmol/kg/min. Increasing concentrations of L-histidine enhanced 65Zn flux into brain at [Zn2+] values between 1 and 1,000 nM. The combined effect of [histidine] and [Zn2+] was best accounted for by a function of [ZnHis+], i.e., flux = 64.4.[ZnHis+]/(390 + [ZnHis+]) + 0.00378.[ZnHis+], with concentrations being nanomolar. D-Histidine had an influence similar to that of L-histidine. 65Zn flux in the presence of 100 microM L-histidine was not affected by either 500 microM L-arginine or 500 microM L-phenylanine. The results indicate specific transport of Zn2+ across the plasma membranes of brain endothelium. The enhancement due to histidine has been attributed to diffusion of ZnHis+ across unstirred layers "ferrying" zinc to and from transport sites.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0022-3042
pubmed:author
pubmed:issnType
Print
pubmed:volume
62
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
665-72
pubmed:dateRevised
2010-8-25
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Transport of zinc-65 at the blood-brain barrier during short cerebrovascular perfusion in the rat: its enhancement by histidine.
pubmed:affiliation
Biomedical Sciences Division (Physiology), King's College, London, England, UK.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't