Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1994-3-2
pubmed:abstractText
We have developed a colony papillation assay for monitoring the copy number of genetically marked chromosomes II and III in Saccharomyces cerevisiae. The unique feature of this assay is that it allows detection of a gain of the marked chromosomes even if there is a gain of the entire set of chromosomes (increase-in-ploidy). This assay was used to screen for chromosome-gain or increase-in-ploidy mutants. Five complementation groups have been defined for recessive mutations that confer an increase-in-ploidy (ipl) phenotype, which, in each case, cosegregates with a temperature-sensitive growth phenotype. Four new alleles of CDC31, which is required for spindle pole body duplication, were also recovered from this screen. Temperature-shift experiments with ipl1 cells show that they suffer severe nondisjunction at 37 degrees. Similar experiments with ipl2 cells show that they gain entire sets of chromosomes and become arrested as unbudded cells at 37 degrees. Molecular cloning and genetic mapping show that IPL1 is a newly identified gene, whereas IPL2 is allelic to BEM2, which is required for normal bud growth.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/8293973-1092597, http://linkedlifedata.com/resource/pubmed/commentcorrection/8293973-1341195, http://linkedlifedata.com/resource/pubmed/commentcorrection/8293973-1493337, http://linkedlifedata.com/resource/pubmed/commentcorrection/8293973-1538785, http://linkedlifedata.com/resource/pubmed/commentcorrection/8293973-1581964, http://linkedlifedata.com/resource/pubmed/commentcorrection/8293973-1618897, http://linkedlifedata.com/resource/pubmed/commentcorrection/8293973-1651171, http://linkedlifedata.com/resource/pubmed/commentcorrection/8293973-1651172, http://linkedlifedata.com/resource/pubmed/commentcorrection/8293973-17176533, http://linkedlifedata.com/resource/pubmed/commentcorrection/8293973-17248617, http://linkedlifedata.com/resource/pubmed/commentcorrection/8293973-1860883, http://linkedlifedata.com/resource/pubmed/commentcorrection/8293973-1869587, http://linkedlifedata.com/resource/pubmed/commentcorrection/8293973-1905981, http://linkedlifedata.com/resource/pubmed/commentcorrection/8293973-1992340, http://linkedlifedata.com/resource/pubmed/commentcorrection/8293973-1996092, http://linkedlifedata.com/resource/pubmed/commentcorrection/8293973-2010084, http://linkedlifedata.com/resource/pubmed/commentcorrection/8293973-2195038, http://linkedlifedata.com/resource/pubmed/commentcorrection/8293973-2403635, http://linkedlifedata.com/resource/pubmed/commentcorrection/8293973-2404021, http://linkedlifedata.com/resource/pubmed/commentcorrection/8293973-2407610, http://linkedlifedata.com/resource/pubmed/commentcorrection/8293973-2538717, http://linkedlifedata.com/resource/pubmed/commentcorrection/8293973-2657385, http://linkedlifedata.com/resource/pubmed/commentcorrection/8293973-2678811, http://linkedlifedata.com/resource/pubmed/commentcorrection/8293973-3030557, http://linkedlifedata.com/resource/pubmed/commentcorrection/8293973-3036373, http://linkedlifedata.com/resource/pubmed/commentcorrection/8293973-3044610, http://linkedlifedata.com/resource/pubmed/commentcorrection/8293973-3072479, http://linkedlifedata.com/resource/pubmed/commentcorrection/8293973-3116672, http://linkedlifedata.com/resource/pubmed/commentcorrection/8293973-3290223, http://linkedlifedata.com/resource/pubmed/commentcorrection/8293973-3510081, http://linkedlifedata.com/resource/pubmed/commentcorrection/8293973-3526331, http://linkedlifedata.com/resource/pubmed/commentcorrection/8293973-3540592, http://linkedlifedata.com/resource/pubmed/commentcorrection/8293973-3540600, http://linkedlifedata.com/resource/pubmed/commentcorrection/8293973-375221, http://linkedlifedata.com/resource/pubmed/commentcorrection/8293973-378116, http://linkedlifedata.com/resource/pubmed/commentcorrection/8293973-388424, http://linkedlifedata.com/resource/pubmed/commentcorrection/8293973-3885221, http://linkedlifedata.com/resource/pubmed/commentcorrection/8293973-3889913, http://linkedlifedata.com/resource/pubmed/commentcorrection/8293973-3894160, http://linkedlifedata.com/resource/pubmed/commentcorrection/8293973-3967296, http://linkedlifedata.com/resource/pubmed/commentcorrection/8293973-5419266, http://linkedlifedata.com/resource/pubmed/commentcorrection/8293973-6273866, http://linkedlifedata.com/resource/pubmed/commentcorrection/8293973-6329902, http://linkedlifedata.com/resource/pubmed/commentcorrection/8293973-6330094, http://linkedlifedata.com/resource/pubmed/commentcorrection/8293973-6336730, http://linkedlifedata.com/resource/pubmed/commentcorrection/8293973-6757720, http://linkedlifedata.com/resource/pubmed/commentcorrection/8293973-7019215, http://linkedlifedata.com/resource/pubmed/commentcorrection/8293973-7028565, http://linkedlifedata.com/resource/pubmed/commentcorrection/8293973-7035289, http://linkedlifedata.com/resource/pubmed/commentcorrection/8293973-7046050, http://linkedlifedata.com/resource/pubmed/commentcorrection/8293973-8349727
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0016-6731
pubmed:author
pubmed:issnType
Print
pubmed:volume
135
pubmed:geneSymbol
BEM2, CDC31, ILP2, IPL1
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
677-91
pubmed:dateRevised
2010-9-13
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Isolation and characterization of chromosome-gain and increase-in-ploidy mutants in yeast.
pubmed:affiliation
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.
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