Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1994-2-28
|
| pubmed:abstractText |
The beta-adrenergic receptor system of the failing human heart is markedly desensitized. We have recently postulated that this desensitization may in part be caused by an increase in beta-adrenergic receptor kinase (beta ARK) expression. beta ARK is thought to effect desensitization by acting in concert with an inhibitor protein, called beta-arrestin. Two isoforms have been identified both for beta ARK and for beta-arrestin. In the present study, we have investigated the expression of the individual isoforms of beta-arrestin and of beta ARK in left ventricles from failing and control human hearts. mRNAs for all four proteins, beta-arrestin-1, beta-arrestin-2, beta ARK-1, and beta ARK-2, were identified in human heart. Quantitation by reverse-transcription polymerase chain reactions showed that in heart failure there were no changes of the mRNA levels for beta-arrestin-1 and beta-arrestin-2, a slight (< 50%) increase of the mRNA for beta ARK-2, and a threefold increase for beta ARK-1 mRNA. At the protein level, beta-arrestin-1 was readily detected by Western blotting in human heart. Its absolute values were approximately 350 fmol/mg cytosolic protein, and its expression was not changed in heart failure. beta-Arrestin-2 levels were too low to be detectable using the same methods. beta ARK levels as determined by enzymatic activity were approximately 20 fmol/mg cytosolic protein (beta ARK-1 plus beta ARK-2) and thus almost 20-fold lower than those of beta-arrestin. beta ARK levels were increased approximately twofold in heart failure.(ABSTRACT TRUNCATED AT 250 WORDS)
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/Arrestins,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP-Dependent Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Eye Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/beta-Adrenergic Receptor Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/beta-arrestin
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
0009-7330
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
74
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
206-13
|
| pubmed:dateRevised |
2007-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:8293560-Adult,
pubmed-meshheading:8293560-Aged,
pubmed-meshheading:8293560-Amino Acid Sequence,
pubmed-meshheading:8293560-Antigens,
pubmed-meshheading:8293560-Arrestins,
pubmed-meshheading:8293560-Blotting, Western,
pubmed-meshheading:8293560-Cardiac Output, Low,
pubmed-meshheading:8293560-Cyclic AMP-Dependent Protein Kinases,
pubmed-meshheading:8293560-Eye Proteins,
pubmed-meshheading:8293560-Female,
pubmed-meshheading:8293560-Humans,
pubmed-meshheading:8293560-Isomerism,
pubmed-meshheading:8293560-Male,
pubmed-meshheading:8293560-Middle Aged,
pubmed-meshheading:8293560-Molecular Sequence Data,
pubmed-meshheading:8293560-Myocardium,
pubmed-meshheading:8293560-Peptide Fragments,
pubmed-meshheading:8293560-Polymerase Chain Reaction,
pubmed-meshheading:8293560-RNA, Messenger,
pubmed-meshheading:8293560-Transcription, Genetic,
pubmed-meshheading:8293560-beta-Adrenergic Receptor Kinases
|
| pubmed:year |
1994
|
| pubmed:articleTitle |
Expression of beta-arrestins and beta-adrenergic receptor kinases in the failing human heart.
|
| pubmed:affiliation |
Laboratorium für Molekulare Biologie, Universität München, Max-Planck-Institut für Biochemie, Martinsried, Germany.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|