Linked Life Data

8292973

Source:http://linkedlifedata.com/resource/pubmed/id/8292973

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3-4
pubmed:dateCreated
1994-2-28
pubmed:abstractText
In this paper we show the presence of 2-[125I]melatonin binding sites in human neutrophils (hN). The specific binding of melatonin to hN cells and hN membranes was dependent on time and temperature, stable, saturable, and reversible. In competition studies, the specific binding of radioactive melatonin to hN cells or hN membranes was inhibited by increasing concentrations of native melatonin. Scatchard analysis showed the existence of a single class of binding sites with a Kd of 2.1 and 7.1 microM for hN cells and hN membranes, respectively. The binding capacity was of 84 and 132 pM for hN cells and hN membranes, respectively. The affinity of the binding sites for melatonin suggests that they may be relevant in studies on the pharmacological properties of melatonin in regulating human neutrophil activity.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0898-2848
pubmed:author
pubmed:issnType
Print
pubmed:volume
4
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
253-62
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Characterization of melatonin binding sites in human peripheral blood neutrophils.
pubmed:affiliation
University of Seville School of Medicine, Spain.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't