Linked Life Data

8291511

Source:http://linkedlifedata.com/resource/pubmed/id/8291511

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1994-2-18
pubmed:abstractText
The present experiments tested the hypothesis that decreased production of an endothelium-derived relaxing factor in arteries of hypertensive animals contributes to impaired endothelium-dependent relaxation. Acetylcholine-induced relaxation of freshly isolated spontaneously hypertensive rat (SHR) vessels pre-contracted with norepinephrine was impaired compared with Wistar-Kyoto rats (WKY). 10 microM indomethacin partially normalized the SHR response. Inhibition of nitric oxide synthesis with 100 microM nitro-L-arginine methyl ester or 100 microM NG-monomethyl arginine shifted the acetylcholine relaxation curve to the right, but had no effect on the maximal relaxation in WKY and completely inhibited relaxation of SHR. A similar pattern was observed with methylene blue (0.3 microM). Acetylcholine-induced relaxation of WKY vessels pre-contracted with 5 microM norepinephrine and 100 mM K+ was attenuated 60% compared with vessels pre-contracted with norepinephrine alone, and was completely inhibited by nitro-L-arginine methyl ester; relaxation of SHR vessels was decreased by 50%. Six-hour storage at 37 degrees C significantly attenuated acetylcholine-induced relaxation in both strains; treatment with indomethacin improved the response. Moreover, relaxation of WKY vessels was completely inhibited by nitro-L-arginine methyl ester and NG-monomethyl arginine after the storage period. The absence of L-arginine-induced relaxation and lack of effect of supplementation with L-arginine indicated that the constitutively active NO synthetase was not induced and that L-arginine substrate was limiting. The results indicate that mesenteric resistance arteries of the WKY express a relaxing factor generated by a non-cyclo-oxygenase, non-nitric oxide synthetase pathway that is possibly a hyperpolarizing factor.(ABSTRACT TRUNCATED AT 250 WORDS)
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0002-9629
pubmed:author
pubmed:issnType
Print
pubmed:volume
307
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
7-14
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
A non-cyclo-oxygenase, non-nitric oxide relaxing factor is present in resistance arteries of normotensive but not spontaneously hypertensive rats.
pubmed:affiliation
Hypertension and Vascular Research, Department of Internal Medicine, University of Texas Medical Branch, Galveston 77550.
pubmed:publicationType
Journal Article, In Vitro, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't