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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1994-2-24
|
| pubmed:abstractText |
To analyze the proteins produced from the rubella virus (RUB) nonstructural protein open reading frame (NSP-ORF), a DNA containing the RUB NSP-ORF was introduced into the expression vector pTM3 in which the sequences to be expressed are downstream from a T7 RNA polymerase promoter. In cells infected with a vaccinia virus recombinant which expresses T7 RNA polymerase and transfected with this plasmid, three RUB-specific products with electrophoretic mobilities of 200, 150, and 97 kDa were clearly visible. By computer alignment, the presence of a cysteine protease was predicted within the NSP-ORF (A. E. Gorbalenya et al., FEBS Lett. 288, 201-205, 1991). When the Cys proposed as the catalytic residue of this protease (Cys1151) was mutated to a Gly, only the 200-kDa product was produced, demonstrating that the Cys is important in the activity of the protease responsible for the processing of the RUB NSPs and that the 150- and 97-kDa species are processing products. Transfections with deletion mutants revealed that the 150-kDa processing product is derived from the amino-terminal two-thirds of the ORF and that both the protease and the cleavage site on the COOH-terminal side of the 150-kDa product are between amino acids 1005 and 1507 of the ORF.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
0042-6822
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
198
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
586-92
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:8291241-Animals,
pubmed-meshheading:8291241-Base Sequence,
pubmed-meshheading:8291241-Cells, Cultured,
pubmed-meshheading:8291241-Cloning, Molecular,
pubmed-meshheading:8291241-Cricetinae,
pubmed-meshheading:8291241-Cysteine Endopeptidases,
pubmed-meshheading:8291241-DNA, Viral,
pubmed-meshheading:8291241-DNA Mutational Analysis,
pubmed-meshheading:8291241-Molecular Sequence Data,
pubmed-meshheading:8291241-Mutagenesis, Site-Directed,
pubmed-meshheading:8291241-Open Reading Frames,
pubmed-meshheading:8291241-Protein Processing, Post-Translational,
pubmed-meshheading:8291241-Recombinant Proteins,
pubmed-meshheading:8291241-Rubella virus,
pubmed-meshheading:8291241-Sequence Deletion,
pubmed-meshheading:8291241-Sequence Homology, Amino Acid,
pubmed-meshheading:8291241-Vaccinia virus,
pubmed-meshheading:8291241-Viral Nonstructural Proteins
|
| pubmed:year |
1994
|
| pubmed:articleTitle |
Expression of the rubella virus nonstructural protein ORF and demonstration of proteolytic processing.
|
| pubmed:affiliation |
Department of Biology, Georgia State University, Atlanta 30302-4010.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|