|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
2
|
|
pubmed:dateCreated |
1994-2-22
|
|
pubmed:databankReference |
|
|
pubmed:abstractText |
We used 5-azido-[7-3H]indole-3-acetic acid (5-azido-[7-3H]IAA), a photoaffinity analogue of the plant hormone indole-3-acetic acid (IAA), to search for auxin-binding proteins in Arabidopsis thaliana membranes. We identified an auxin-binding protein with a molecular mass of 24 kDa (Atpm24) in microsomes as well as in plasma membrane vesicles. Atpm24 was solubilized by 1% Triton X-100 and partially purified. A cDNA clone (Atpm24.1) corresponding to Atpm24 was isolated. The amino acid sequence predicted from the Atpm24.1 cDNA contains 212 amino acid residues with a relative molecular mass of 24,128 Da. Data base searches revealed that the predicted protein has homology to glutathione S-transferases (GSTs; EC 2.5.1.18). When Atpm24.1 was expressed in Escherichia coli, we found a high level of GST activity in the bacterial extracts. We have analyzed the substrate specificity of this protein and found that cumene hydroperoxide and trans-stilbene oxide but not trans-cinnamic acid or IAA-CoA were substrates. A role for this GST in physiological processes of plants is discussed.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8290582-11537412,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8290582-11537490,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8290582-11607251,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8290582-1522586,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8290582-16594060,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8290582-1729717,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8290582-1799693,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8290582-1850406,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8290582-2065650,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8290582-2199796,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8290582-3069329,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8290582-3277162,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8290582-3372534,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8290582-6301011,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8290582-666896,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8290582-6884349,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8290582-6989596,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8290582-7173206,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8290582-8108497,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8290582-8329687
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/5-azido-1H-indole-3-acetic acid,
http://linkedlifedata.com/resource/pubmed/chemical/Affinity Labels,
http://linkedlifedata.com/resource/pubmed/chemical/Azides,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/Glutathione Transferase,
http://linkedlifedata.com/resource/pubmed/chemical/Indoleacetic Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Plant Growth Regulators,
http://linkedlifedata.com/resource/pubmed/chemical/Plant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cell Surface,
http://linkedlifedata.com/resource/pubmed/chemical/auxin receptor, plant
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jan
|
|
pubmed:issn |
0027-8424
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
18
|
|
pubmed:volume |
91
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
689-93
|
|
pubmed:dateRevised |
2010-9-13
|
|
pubmed:meshHeading |
pubmed-meshheading:8290582-Affinity Labels,
pubmed-meshheading:8290582-Amino Acid Sequence,
pubmed-meshheading:8290582-Arabidopsis,
pubmed-meshheading:8290582-Azides,
pubmed-meshheading:8290582-Base Sequence,
pubmed-meshheading:8290582-Cell Membrane,
pubmed-meshheading:8290582-Cloning, Molecular,
pubmed-meshheading:8290582-DNA Primers,
pubmed-meshheading:8290582-Escherichia coli,
pubmed-meshheading:8290582-Glutathione Transferase,
pubmed-meshheading:8290582-Indoleacetic Acids,
pubmed-meshheading:8290582-Molecular Sequence Data,
pubmed-meshheading:8290582-Molecular Weight,
pubmed-meshheading:8290582-Plant Growth Regulators,
pubmed-meshheading:8290582-Plant Proteins,
pubmed-meshheading:8290582-Receptors, Cell Surface,
pubmed-meshheading:8290582-Sequence Homology, Amino Acid,
pubmed-meshheading:8290582-Substrate Specificity
|
|
pubmed:year |
1994
|
|
pubmed:articleTitle |
Photoaffinity labeling of Arabidopsis thaliana plasma membrane vesicles by 5-azido-[7-3H]indole-3-acetic acid: identification of a glutathione S-transferase.
|
|
pubmed:affiliation |
Max-Planck-Institut für Züchtungsforschung, Cologne, Federal Republic of Germany.
|
|
pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|
