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rdf:type |
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lifeskim:mentions |
umls-concept:C0040648,
umls-concept:C0086860,
umls-concept:C0108685,
umls-concept:C0205314,
umls-concept:C0304925,
umls-concept:C0679622,
umls-concept:C1420631,
umls-concept:C1514562,
umls-concept:C1515877,
umls-concept:C1551336,
umls-concept:C1879547,
umls-concept:C1880389,
umls-concept:C1883204,
umls-concept:C1883221,
umls-concept:C2003941
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pubmed:issue |
2
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pubmed:dateCreated |
1994-2-22
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pubmed:abstractText |
HNF1 and C/EBP alpha are transcription factors that bind to and trans-activate the human albumin gene proximal promoter. Various 5' deletions of the human albumin promoter were coupled to a luciferase reporter gene (alb-luc constructs) and co-electroporated with HNF1 and/or C/EBP alpha expression vectors into HeLa cells. Luciferase activities from co-electroporation of the HNF1 and C/EBP alpha expression vectors with the alb-luc constructs were approximately 10-fold greater than the sum of the activities achieved with HNF1 and C/EBP alpha alone. Analysis of COOH-terminal or internal deletions of the HNF1 expression vector revealed that the domain important for collaborative interaction with C/EBP alpha could be localized to a 157 amino acid region not previously described. This domain is proline and glutamine-rich and is highly homologous (66%) to a portion of vHNF1, an evolutionarily related gene first identified in dedifferentiated hepatoma cells. A construct linking the negatively charged activation domain of herpes simplex virus protein VP16 to the DNA-binding domain of HNF1 showed that it could also synergize with C/EBP alpha to trans-activate the human albumin gene promoter. Our studies delineate a domain in HNF1 important for synergistic activation with C/EBP alpha.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Albumins,
http://linkedlifedata.com/resource/pubmed/chemical/CCAAT-Enhancer-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/HNF1A protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/HNF1B protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Hepatocyte Nuclear Factor 1,
http://linkedlifedata.com/resource/pubmed/chemical/Hepatocyte Nuclear Factor 1-alpha,
http://linkedlifedata.com/resource/pubmed/chemical/Hepatocyte Nuclear Factor 1-beta,
http://linkedlifedata.com/resource/pubmed/chemical/Herpes Simplex Virus Protein Vmw65,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
14
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pubmed:volume |
269
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pubmed:geneSymbol |
C/EBP,
GAL4,
GCN4,
HNF1,
Rta(2a),
Sp1,
VP16
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1177-82
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:8288579-Albumins,
pubmed-meshheading:8288579-Amino Acid Sequence,
pubmed-meshheading:8288579-Binding Sites,
pubmed-meshheading:8288579-CCAAT-Enhancer-Binding Proteins,
pubmed-meshheading:8288579-DNA-Binding Proteins,
pubmed-meshheading:8288579-Drug Synergism,
pubmed-meshheading:8288579-Gene Expression Regulation,
pubmed-meshheading:8288579-Hepatocyte Nuclear Factor 1,
pubmed-meshheading:8288579-Hepatocyte Nuclear Factor 1-alpha,
pubmed-meshheading:8288579-Hepatocyte Nuclear Factor 1-beta,
pubmed-meshheading:8288579-Herpes Simplex Virus Protein Vmw65,
pubmed-meshheading:8288579-Humans,
pubmed-meshheading:8288579-Nuclear Proteins,
pubmed-meshheading:8288579-Promoter Regions, Genetic,
pubmed-meshheading:8288579-Recombinant Proteins,
pubmed-meshheading:8288579-Sequence Alignment,
pubmed-meshheading:8288579-Sequence Homology, Amino Acid,
pubmed-meshheading:8288579-Transcription, Genetic,
pubmed-meshheading:8288579-Transcription Factors,
pubmed-meshheading:8288579-Transcriptional Activation
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pubmed:year |
1994
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pubmed:articleTitle |
The transcription factor HNF1 acts with C/EBP alpha to synergistically activate the human albumin promoter through a novel domain.
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pubmed:affiliation |
Department of Cell Biology, Baylor College of Medicine, Houston, Texas 77030.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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