Linked Life Data

8288158

Source:http://linkedlifedata.com/resource/pubmed/id/8288158

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
1994-2-24
pubmed:abstractText
Prolactin (PRL) was found to stimulate protein kinase C (PKC) activity in a transient fashion in isolated nuclei derived from the mammary glands of 12-14 day pregnant mice. PKC activation was time and dose dependent and was blocked by staurosporine. With 10 ng/ml PRL a maximum stimulation of PKC occurred at 3 min, whereas with 50 ng/ml the effect was maximal at 2 min. After 5 min, the effect of PRL on PKC activity was no longer detected. Specificity of the PRL effect on PKC was established by showing that bovine growth hormone and insulin at 10 ng/ml had no effect on PKC activity. Multiple proteins in the nuclear preparations were shown to be phosphorylated by the addition of PKC derived from rat brain tissue. These studies have important implications regarding the possible direct effects of prolactin in the nucleus of mammary cells.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0018-5043
pubmed:author
pubmed:issnType
Print
pubmed:volume
25
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
564-8
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Prolactin stimulation of protein kinase C in isolated mouse mammary gland nuclei.
pubmed:affiliation
Department of Physiology, Wayne State University School of Medicine, Detroit, Michigan.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.