|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
2
|
|
pubmed:dateCreated |
1994-2-18
|
|
pubmed:abstractText |
We have suggested a random modification method for determining preferable binding sites of a DNA-binding protein and applied this method to the Oct-2B transcription factor. Our results indicate that the Oct-2B protein interacts with canonical oct sequence ATGC/TAAAT and degenerated sequences which contain TAAT motif in the binding site. We have determined nucleotides in the binding sites, involved in the DNA-protein interaction, and the equilibrium dissociation constants Kd for these sequences. These data show that a much greater number of potential targets for Oct proteins exist on DNA and changed our view on the gene expression regulation by this protein factor.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jan
|
|
pubmed:issn |
0014-5793
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
10
|
|
pubmed:volume |
337
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
175-8
|
|
pubmed:dateRevised |
2006-11-15
|
|
pubmed:meshHeading |
pubmed-meshheading:8287972-Animals,
pubmed-meshheading:8287972-Base Sequence,
pubmed-meshheading:8287972-Binding Sites,
pubmed-meshheading:8287972-Cell Line,
pubmed-meshheading:8287972-Cloning, Molecular,
pubmed-meshheading:8287972-DNA-Binding Proteins,
pubmed-meshheading:8287972-Methylation,
pubmed-meshheading:8287972-Mice,
pubmed-meshheading:8287972-Molecular Sequence Data,
pubmed-meshheading:8287972-Octamer Transcription Factor-2,
pubmed-meshheading:8287972-Oligodeoxyribonucleotides,
pubmed-meshheading:8287972-Recombinant Proteins,
pubmed-meshheading:8287972-Transcription Factors
|
|
pubmed:year |
1994
|
|
pubmed:articleTitle |
Noncanonical Oct-sequences are targets for mouse Oct-2B transcription factor.
|
|
pubmed:affiliation |
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
